{"id":331,"date":"2023-02-23T12:27:02","date_gmt":"2023-02-23T12:27:02","guid":{"rendered":"https:\/\/yunshangtulv.com.cn\/?p=331"},"modified":"2023-02-23T12:27:02","modified_gmt":"2023-02-23T12:27:02","slug":"%e7%bb%86%e8%83%9e%e6%af%92%e5%ae%9e%e9%aa%8c%ef%bc%9a%e8%8d%af%e7%89%a9%e6%a2%af%e5%ba%a6%e8%ae%be%e7%bd%ae%e5%92%8c%e8%8d%af%e7%89%a9%e9%85%8d%e6%af%94%e8%ae%a1%e7%ae%97","status":"publish","type":"post","link":"https:\/\/yunshangtulv.com.cn\/?p=331","title":{"rendered":"\u7ec6\u80de\u6bd2\u5b9e\u9a8c\uff1a\u836f\u7269\u68af\u5ea6\u8bbe\u7f6e\u548c\u836f\u7269\u914d\u6bd4\u8ba1\u7b97"},"content":{"rendered":"

\u8ba4\u8bc6\u5355\u4f4d<\/h2>\n

\u4f53\u79ef\u6469\u5c14\u6d53\u5ea6\uff08\u5373<\/span>\u7269\u8d28\u7684\u91cf\u6d53\u5ea6\uff09\u53ef\u7b80\u5199\u4e3a M<\/span> <\/span><\/sup>\uff0c\u8868\u793amol\/L<\/span><\/p>\n

nM (nmol\/L)<\/span><\/p>\n

\u03bcM (\u03bcmol\/L)<\/span><\/p>\n

mM (mmol\/L)<\/span><\/p>\n

M (mol\/L)<\/span><\/p>\n

1 \u03bcM=1000 nM<\/div>\n
1 mM=1000 \u03bcM<\/div>\n
1 M=1000 mM<\/div>\n
1 M=1 mol\/L = 1 mol\/dm\u00b3<\/div>\n
\n
\u5728\u6469\u5c14\u8ba1\u7b97\u4e2d\uff0c\u4f53\u79ef\u6469\u5c14\u6d53\u5ea6(Molarity)\u548c\u8d28\u91cf\u6469\u5c14\u6d53\u5ea6(Molality)\u662f\u4e0d\u4e00\u6837\u7684\u3002<\/div>\n
\u4f53\u79ef\u6469\u5c14\u6d53\u5ea6\u5355\u4f4d\u4e3amol\/L\uff0c\u800c\u8d28\u91cf\u6469\u5c14\u6d53\u5ea6\u5355\u4f4d\u662fmol\/kg<\/div>\n<\/div>\n

\u5b9e\u9a8c\u51c6\u5907<\/h2>\n

\u67e5\u6587\u732e\uff1a\u836f\u7269IC50\u503c\uff08\u598210\u03bcM<\/span>\uff09<\/p>\n

\u8bbe\u7f6e\u6d53\u5ea6\u68af\u5ea6<\/h3>\n

\u836f\u7269\u7684\u6d53\u5ea6\uff08\u7ec8\u6d53\u5ea6\uff09\u4e00\u822c\u5e94\u5728nM\uff5e\u03bcM\u7ea7\uff0c\u800c\u6bcd\u6db2\u5e38\u89c4\u662f10\uff5e30 mM\uff08in DMSO\uff09<\/span><\/p>\n

\u836f\u7269\u6d53\u5ea6\u68af\u5ea6\u70b9\u4e3a6\uff5e10\u4e2a\u4e3a\u5b9c\uff08\u5305\u542b0\u6d53\u5ea6\uff09\uff1b<\/span><\/p>\n

\u8fc7\u5c11\uff1a<\/strong>\u53ef\u80fd\u7ed8\u5236\u4e0d\u51fa\u597d\u7684\u7ebf<\/span><\/p>\n

\u8fc7\u591a\uff1a<\/strong>96\u5b54\u677f\u4e0d\u5b9c\u5b89\u6392\u6392\u5e03, \u914d\u836f\u52a0\u836f\u5bb9\u6613\u51fa\u9519\u3002<\/span><\/p>\n

\u5e38\u89c1\u6d53\u5ea6\u68af:<\/strong>2\u30013\u30015\u3001\u621610\uff0c\u4ee53\u4e3a\u6700\u5e38\u7528<\/strong><\/p>\n

2\u500d\uff1a<\/strong><\/p>\n

0, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 \u03bcM;<\/p>\n

0, 1, 2.5, 5, 10, 25, 50, 100, 250 nM;<\/p>\n

3\u500d\uff1a<\/strong><\/p>\n

0, 0.3, 1, 3, 10, 30, 100, 300, 1000 nM;<\/p>\n

5\u500d\uff1a<\/strong><\/p>\n

0, 1.6, 8, 40, 200, 1000, 5000 nM;<\/p>\n

10\u500d\uff1a<\/strong><\/p>\n

0, 0.004, 0.04, 0.4, 4, 40, 400 \u03bcM;<\/p>\n

\u5b9e\u9a8c\u8bbe\u8ba1\u5efa\u8bae\uff1a<\/strong>\u505a\u4e24\u8f6e\u68af\u5ea6\u5b9e\u9a8c\uff0c\u7b2c\u4e00\u8f6e\u7c97\uff0c\u7b2c\u4e8c\u8f6e\u7ec6\u3002<\/p>\n

\u7b2c\u4e00\u8f6e\u4f4e\u70b9\u8981\u5728\u6700\u4f4e\u53c2\u8003IC50\u76841\/50\u4ee5\u4e0b\uff0c\u9ad8\u70b9\u81f3\u5c11\u8981\u5728\u6700\u9ad8\u53c2\u8003IC50\u768450\u500d\u4ee5\u4e0a\u3002<\/p>\n

\u7b2c\u4e8c\u8f6e\u7b49\u6bd4\u6570\u5217\u53ef\u4ee5\u7f29\u5c0f\u4e00\u7ea7\uff0c\u6216\u8005\u5728\u7b2c\u4e00\u8f6e\u7684IC50\u533a\u95f4\u589e\u52a0\u5bc6\u5ea6\uff0c\u4f46\u6ce8\u610f\u7b2c\u4e8c\u8f6e\u4e0d\u80fd\u592a\u7ec6\uff0c\u6216\u8005\u533a\u95f4\u592a\u7a84\u3002<\/p>\n

\u836f\u7269\u914d\u6bd4\uff0896\u5b54\u677f\uff09<\/h2>\n

\u5df2\u77e5\u6761\u4ef6<\/strong><\/h3>\n

\u5e94\u7528\u6db2\uff1a1nmol\/\u03bcL=1mM<\/span><\/strong><\/p>\n

\u76f8\u5173\u6587\u732e\u663e\u793a\u5728\u7ec6\u80de\u4e2dIC50\u4e3a10\u03bcM<\/span><\/p>\n

96\u5b54\u677f<\/span><\/p>\n

\u91cd\u590d<\/span>6\u590d\u5b54<\/p>\n

6\u4e2a\u68af\u5ea6<\/p>\n

\u5b9e\u9a8c\u51c6\u5907<\/h3>\n

\u6d53\u5ea6\u68af\u5ea6\u5206\u7ec4\uff1a0\u03bcM\u00a0 2.5\u03bcM\u00a0 5\u03bcM\u00a0\u00a0<\/span>10\u03bcM<\/span><\/strong>\u00a0 20\u03bcM\u00a0 40\u03bcM<\/span><\/p>\n

\u7269\u54c1\u51c6\u5907\uff1a\u914d\u5236\u597d\u7684\u836f\u7269\u300196\u5b54\u677f\u57f9\u517b\u7684\u5df2\u8d34\u58c1\u7ec6\u80de\u3001\u79fb\u6db2\u67aa\u3001\u914d\u5bf9\u67aa\u5934\u3001\u57f9\u517b\u57fa\u7b49<\/p>\n

\u8ba1\u7b97\u914d\u836f<\/h3>\n

\u8bbe\u5b9a<\/h4>\n

\u6bcf\u5b54\u4e2d\u5df2\u542b\u6709100ul 10%FBS\u57f9\u517b\u57fa<\/p>\n

\u6bcf\u5b54\u4e2d\u52a0\u5165100ul\u836f\u7269\u6eb6\u6db2<\/p>\n

\u500d\u6bd4\u7a00\u91ca<\/h4>\n\n\n\n\n\n\n\n\n\n
<\/td>\n\u5b54<\/td>\n<\/tr>\n
40\u03bcM<\/td>\n100\u03bcl\u539f\u57f9\u517b\u6db2+100\u03bcl80\u03bcM\u836f\u7269\u6eb6\u6db2=200\u03bcl40\u03bcM<\/td>\n<\/tr>\n
20\u03bcM<\/td>\n100\u03bcl\u539f\u57f9\u517b\u6db2+100\u03bcl40\u03bcM\u836f\u7269\u6eb6\u6db2=200\u03bcl20\u03bcM<\/td>\n<\/tr>\n
10\u03bcM<\/td>\n100\u03bcl\u539f\u57f9\u517b\u6db2+100\u03bcl20\u03bcM\u836f\u7269\u6eb6\u6db2=200\u03bcl10\u03bcM<\/td>\n<\/tr>\n
5\u03bcM<\/td>\n100\u03bcl\u539f\u57f9\u517b\u6db2+100\u03bcl10\u03bcM\u836f\u7269\u6eb6\u6db2=200\u03bcl5\u03bcM<\/td>\n<\/tr>\n
2.5\u03bcM<\/td>\n100\u03bcl\u539f\u57f9\u517b\u6db2+100\u03bcl5\u03bcM\u836f\u7269\u6eb6\u6db2=200\u03bcl2.5\u03bcM<\/td>\n<\/tr>\n
0\u03bcM<\/td>\n200\u03bcl\u57f9\u517b\u6db2=200\u03bcl0\u03bcM<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5e94\u7528\u6db2\u6d53\u5ea6\uff1a1mM<\/strong><\/p>\n

\u8fd0\u7b97\uff1a<\/strong>1mM=1000\u03bcM\uff1b1000\u03bcM\/80\u03bcM=12.5\u500d<\/p>\n

\u5373\uff1a\u5c06\u5e94\u7528\u6db2<\/span>\u7a00\u91ca12.5\u500d<\/strong><\/span><\/p>\n

\u914d\u5236\u6700\u5927\u836f\u7269\u6d53\u5ea6\u4e24\u500d\u7684\u6d53\u5ea6\uff1a80\u03bcM<\/strong><\/p>\n

\u53d6<\/span>96\u03bcL<\/strong>\u7684<\/span>\u836f\u7269\u5e94\u7528\u6db2\uff0c\u52a0\u4e0a1104\u03bcL<\/strong>\u7684\u65e0\u8840\u6e05\u57f9\u517b\u57fa\uff0c\u5373<\/span>\u5c0696\u03bcL\u7684\u836f\u7269\u5e94\u7528\u6db2\u7a00\u91ca12.5\u500d<\/strong><\/span>\uff0c\u81f3\u7ec8\u4f53\u79ef\u4e3a1.2mL\uff0c\u5373<\/span>\u914d\u5236\u51fa\u4e86<\/span>1200\u03bcL<\/strong><\/span>\u7684<\/span>80\u03bcM<\/strong><\/span>\u7684\u836f\u54c1\u3002<\/span><\/p>\n

40\u03bcM\uff1a\u6bcf\u5b54\u52a0\u5165100\u03bcL\u768480\u03bcM\u7684\u836f\u54c1\uff0c6\u4e2a\u590d\u5b54\u5171600\u03bcL\u6db2\u4f53<\/strong><\/p>\n

\u8865\u5165600\u03bcL\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u5e76\u5439\u5300\uff0c\u5373\u5c06\u5269\u4f59\u836f\u54c1\u6d53\u5ea6\u51cf\u534a\u4e3a40\u03bcM<\/p>\n

20\u03bcM\uff1a\u6bcf\u5b54\u52a0\u5165100\u03bcL\u768440\u03bc<\/span>M\u7684\u836f\u54c1<\/span>\uff0c6\u4e2a\u590d\u5b54\u5171600\u03bcL\u6db2\u4f53<\/span><\/strong><\/p>\n

\u8865\u5165600\u03bcL\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u5e76\u5439\u5300\uff0c\u5373\u5c06\u5269\u4f59\u836f\u54c1\u6d53\u5ea6\u51cf\u534a\u4e3a20\u03bcM<\/p>\n

10\u03bcM\uff1a\u6bcf\u5b54\u52a0\u5165100\u03bcL\u768420\u03bc<\/span>M\u7684\u836f\u54c1<\/span>\uff0c6\u4e2a\u590d\u5b54\u5171600\u03bcL\u6db2\u4f53<\/span><\/strong><\/p>\n

\u8865\u5165600\u03bcL\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u5e76\u5439\u5300\uff0c\u5373\u5c06\u5269\u4f59\u836f\u54c1\u6d53\u5ea6\u51cf\u534a\u4e3a10\u03bcM<\/p>\n

5\u03bcM\uff1a\u6bcf\u5b54\u52a0\u5165100\u03bcL\u768410\u03bc<\/span>M\u7684\u836f\u54c1<\/span>\uff0c6\u4e2a\u590d\u5b54\u5171600\u03bcL\u6db2\u4f53<\/span><\/strong><\/p>\n

\u8865\u5165600\u03bcL\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u5e76\u5439\u5300\uff0c\u5373\u5c06\u5269\u4f59\u836f\u54c1\u6d53\u5ea6\u51cf\u534a\u4e3a5\u03bcM<\/p>\n

2.5\u03bcM\uff1a\u6bcf\u5b54\u52a0\u5165100\u03bcL\u76845\u03bc<\/span>M\u7684\u836f\u54c1<\/span><\/span><\/strong><\/p>\n

\u5b8c\u6210\u52a0\u836f\u5e76<\/span>\u5b75\u80b2\u5230\u8bbe\u5b9a\u65f6\u95f4\u3002<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8ba4\u8bc6\u5355\u4f4d \u4f53\u79ef\u6469\u5c14\u6d53\u5ea6\uff08\u5373\u7269\u8d28\u7684\u91cf\u6d53\u5ea6\uff09\u53ef\u7b80\u5199\u4e3a M \uff0c\u8868\u793amol\/L nM (nmol\/L) \u03bcM (\u03bcmo […]<\/p>\n","protected":false},"author":111,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[],"class_list":["post-331","post","type-post","status-publish","format-standard","hentry","category-10"],"_links":{"self":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/331","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/users\/111"}],"replies":[{"embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=331"}],"version-history":[{"count":0,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/331\/revisions"}],"wp:attachment":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=331"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=331"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=331"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}