{"id":332,"date":"2023-02-23T16:05:10","date_gmt":"2023-02-23T16:05:10","guid":{"rendered":"https:\/\/yunshangtulv.com.cn\/?p=332"},"modified":"2023-02-23T16:09:07","modified_gmt":"2023-02-23T16:09:07","slug":"western-blot","status":"publish","type":"post","link":"https:\/\/yunshangtulv.com.cn\/?p=332","title":{"rendered":"Western blot"},"content":{"rendered":"<p>&nbsp;<\/p>\n<h2><span>\u80cc\u666f\u77e5\u8bc6<\/span><\/h2>\n<h3>\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\uff08\u514d\u75ab\u5370\u8ff9\u8bd5\u9a8c\uff09<\/h3>\n<ul>\n<li>\u7531\u745e\u58eb\u7c73\u6b47\u5c14\u5f17\u96f7\u5fb7\u91cc\u5e0c\u751f\u7269\u7814\u7a76\u6240\uff08Friedrich\u00b7Miescher\u00b7Institute\uff09\u7684Harry\u00b7Towbin\u57281979\u5e74\u63d0\u51fa\u7684\u3002\u5728\u5c3c\u5c14\u00b7\u4f2f\u5948\u7279\uff08Neal\u00b7Burnette\uff09\u4e8e1981\u5e74\u6240\u8457\u7684\u300a\u5206\u6790\u751f\u7269\u5316\u5b66\u300b\uff08Analytical\u00b7Biochemistry\uff09\u4e2d\u9996\u6b21\u88ab\u79f0\u4e3aWestern\u00b7Blot\u3002<\/li>\n<li>\u73b0\u5df2\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u56e0\u5728\u86cb\u767d\u6c34\u5e73\u7684\u8868\u8fbe\u7814\u7a76\u3001\u6297\u4f53\u6d3b\u6027\u68c0\u6d4b\u548c\u75be\u75c5\u65e9\u671f\u8bca\u65ad\u7b49\u591a\u4e2a\u65b9\u9762\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span>\u6297\u4f53\uff08<\/span>antibody<span>\uff09<\/span><\/h3>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677158705472.webp\" \/><\/p>\n<ul>\n<li>1.\u00b7\u6297\u539f\u7ed3\u5408\u533a\uff08Fab\uff09<\/li>\n<li>2.\u00b7<span>\u6297\u539f\u7ed3\u6676\u533a\uff08<\/span>Fc<span>\uff09<\/span><\/li>\n<li>3.\u00b7\u84dd\u8272\u7684\u91cd\u94fe\u6709\u4e00\u4e2a\u53ef\u53d8\u533a\uff08VH\uff09\uff0c\u7d27\u968f\u5176\u540e\u7684\u4e00\u4e2a\u6052\u5b9a\u533a\uff08CH1\uff09\uff0c\u4e00\u4e2a\u67a2\u7ebd\u533a\uff0c\u4ee5\u53ca\u53e6\u4e24\u4e2a\u6052\u5b9a\u533a\uff08CH2\u00b7and\u00b7CH3\uff09\u7ec4\u6210<\/li>\n<li>4.\u00b7\u7eff\u8272\u7684\u8f7b\u94fe\u5305\u542b\u4e00\u4e2a\u53ef\u53d8\u533a\uff08VL\uff09\u4ee5\u53ca\u4e00\u4e2a\u6052\u5b9a\u533a\uff08CL\uff09<\/li>\n<li>5.\u00b7<span>\u6297\u539f\u7ed3\u5408\u70b9<\/span><\/li>\n<li>6.\u00b7<span>\u67a2\u7ebd\u533a<\/span><\/li>\n<\/ul>\n<h2><span>\u5e94\u7528\u8303\u56f4<\/span><\/h2>\n<ul>\n<li><span>\u68c0\u6d4b\u76ee\u7684\u86cb\u767d\u7684\u6297\u539f\u6027<\/span><\/li>\n<li>\u68c0\u6d4b\u76ee\u7684\u86cb\u767d\u7684\u5206\u5b50\u91cf\u5927\u5c0f<\/li>\n<li>\u4ece\u6df7\u5408\u6837\u54c1\u4e2d\u68c0\u6d4b\u76ee\u7684\u86cb\u767d<\/li>\n<li>\u76ee\u7684\u86cb\u767d\u5728\u4e0d\u540c\u6837\u54c1\u4e2d\u7684\u8868\u8fbe\u91cf\u5dee\u5f02<\/li>\n<li>\u7ecf\u8fc7\u4e0d\u540c\u5904\u7406\u540e\uff0c\u76ee\u7684\u86cb\u767d\u7684\u8868\u8fbe\u91cf\u7684\u53d8\u5316<\/li>\n<li>\u786e\u5b9a\u76ee\u7684\u86cb\u767d\u7684\u8868\u8fbe\u60c5\u51b5<\/li>\n<li><span>\u7814\u7a76\u86cb\u767d\u95f4\u7684\u76f8\u4e92\u4f5c\u7528<\/span><\/li>\n<li>\u786e\u5b9a\u76ee\u7684\u86cb\u767d\u7684\u7ec6\u80de\u5b9a\u4f4d<\/li>\n<\/ul>\n<h2><span>\u57fa\u672c\u539f\u7406<\/span><\/h2>\n<h3><span>\u65b9\u6cd5<\/span><\/h3>\n<ul>\n<li><span>\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3<\/span><\/li>\n<\/ul>\n<h3><span>\u88ab\u68c0\u6d4b\u7269<\/span><\/h3>\n<ul>\n<li><span>\u86cb\u767d\u8d28<\/span><\/li>\n<\/ul>\n<h3><span>\u63a2\u9488<\/span><\/h3>\n<ul>\n<li><span>\u6297\u4f53<\/span><\/li>\n<\/ul>\n<h3><span>\u663e\u8272<\/span><\/h3>\n<ul>\n<li><span>\u6807\u8bb0\u7684\u4e8c\u6297<\/span><\/li>\n<\/ul>\n<h3><span>\u8f6c\u819c<\/span><\/h3>\n<ul>\n<li>\u7ecf\u8fc7PAGE\uff08\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff09\u5206\u79bb\u7684\u86cb\u767d\u8d28\u6837\u54c1\uff0c\u8f6c\u79fb\u5230\u56fa\u76f8\u8f7d\u4f53PVDF\u6216NC\u819c\u4e0a\uff0c\u56fa\u76f8\u8f7d\u4f53\u4ee5\u975e\u5171\u4ef7\u952e\u5f62\u5f0f\u5438\u9644\u86cb\u767d\u8d28\uff0c\u4e14\u80fd\u4fdd\u6301\u7535\u6cf3\u5206\u79bb\u7684\u591a\u80bd\u7c7b\u578b\u53ca\u5176\u751f\u7269\u5b66\u6d3b\u6027\u4e0d\u53d8<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span>\u6297\u539f\u6297\u4f53\u53cd\u5e94<\/span><\/h3>\n<ul>\n<li>\u4ee5\u56fa\u76f8\u8f7d\u4f53\u4e0a\u7684\u86cb\u767d\u8d28\u6216\u591a\u80bd\u4f5c\u4e3a\u6297\u539f\uff0c\u4e0e\u5bf9\u5e94\u7684\u6297\u4f53\u8d77\u514d\u75ab\u53cd\u5e94\uff0c\u518d\u4e0e\u9176\u6216\u540c\u4f4d\u7d20\u6807\u8bb0\u7684\u7b2c\u4e8c\u6297\u4f53\u8d77\u53cd\u5e94\uff0c\u7ecf\u8fc7\u5e95\u7269\u663e\u8272\u6216\u653e\u5c04\u81ea\u663e\u5f71\u4ee5\u68c0\u6d4b\u7535\u6cf3\u5206\u79bb\u7684\u7279\u5f02\u6027\u76ee\u7684\u57fa\u56e0\u8868\u8fbe\u7684\u86cb\u767d\u6210\u5206\u3002\u8be5\u6280\u672f\u4e5f\u5e7f\u6cdb\u5e94\u7528\u4e8e\u68c0\u6d4b\u86cb\u767d\u6c34\u5e73\u7684\u8868\u8fbe<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span>\u5185\u53c2<\/span><\/h3>\n<h4><span>\u4f5c\u7528<\/span><\/h4>\n<ul>\n<li>WB<span>\u8fc7\u7a0b\u76d1\u6d4b<\/span><\/li>\n<li><span>\u76ee\u7684\u86cb\u767d\u5b9a\u91cf\u7684\u6807\u51c6<\/span><\/li>\n<\/ul>\n<h4><span>\u610f\u4e49<\/span><\/h4>\n<ul>\n<li>\u6700\u91cd\u8981\u548c\u6700\u4e0d\u53ef\u6216\u7f3a\u7684\u5bf9\u7167<\/li>\n<li>\u53ef\u68c0\u6d4b\u6574\u4e2aWB\u5b9e\u9a8c\u8fc7\u7a0b\u53ca\u4f53\u7cfb\u662f\u5426\u6b63\u5e38\u5de5\u4f5c<\/li>\n<li>\u5982\u679c\u4e00\u4e2a\u5b9e\u9a8c\u8fde\u5185\u53c2\u7167\u90fd\u51fa\u4e0d\u4e86\u9633\u6027\u7ed3\u679c\u7684\u8bdd\uff08\u5728\u5185\u53c2\u6297\u4f53\u6709\u6548\u7684\u60c5\u51b5\u4e0b\uff09,\u90a3\u4e48\u8fd9\u4e2a\u4f53\u7cfb\u5c31\u662f\u5b58\u5728\u95ee\u9898<\/li>\n<\/ul>\n<p><span><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166733500.webp\" \/><\/span><\/p>\n<ul>\n<li><span>\u8d77\u534a\u5b9a\u91cf\u7684\u6807\u51c6\u7684\u4f5c\u7528<\/span><\/li>\n<li>\u5185\u53c2\u4e00\u822c\u9009\u62e9\u7ba1\u5bb6\u57fa\u56e0\u7f16\u7801\u8868\u8fbe\u7684\u86cb\u767d,\u5728\u5f88\u591a\u7ec4\u7ec7\u548c\u7ec6\u80de\u4e2d\u90fd\u662f\u7a33\u5b9a\u8868\u8fbe\u7684,\u56e0\u6b64\u53ef\u4ee5\u4f5c\u4e3a\u68c0\u6d4b\u9776\u86cb\u767d\u8868\u8fbe\u91cf\u7684\u6807\u51c6<\/li>\n<\/ul>\n<h2><span>\u8bbe\u5907\u8017\u6750<\/span><\/h2>\n<h3><span>\u4eea\u5668\u8017\u6750<\/span><\/h3>\n<h3><span>\u5b9e\u9a8c\u836f\u54c1\u8bd5\u5242<\/span><\/h3>\n<h4><span>\u88c2\u89e3\u6db2<\/span><\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166773613.webp\" \/><\/p>\n<ul>\n<li>\u6bcd\u6db2\uff08\u52a8\u7269\u7ec4\u7ec7\u86cb\u767d\u63d0\u53d6Buffer\uff09\uff1a\u86cb\u767d\u9176\u6291\u5236\u5242\uff1aPmsf\uff08\u914d\uff09=100:1:1<\/li>\n<li>\u6bcd\u6db2\u5e38\u6e29\u653e\u7f6e\uff1b\u86cb\u767d\u9176\u6291\u5236\u5242\u548cPmsf\u9700-20\u2103\u4fdd\u5b58\uff1b\u88c2\u89e3\u6db2\u9700-4\u2103\u4fdd\u5b58\u3002<\/li>\n<\/ul>\n<h4><span>\u8003\u9a6c\u65af\u4eae\u84dd\u67d3\u6db2<\/span><\/h4>\n<ul>\n<li>\u4f7f\u7528\u524d\u7528\u65e0\u83cc\u6c34\u7a00\u91ca<\/li>\n<\/ul>\n<h4><span>\u8f6c\u819c\u6db2\u7684\u914d\u5236\uff08<\/span>1*<span>\uff09<\/span>1L<\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166809462.webp\" \/><\/p>\n<ul>\n<li>\u5148\u5b9a\u5bb9800ml\uff0c\u8c03\u5236PH=8.3\uff0c\u7136\u540e+200ml\u7532\u9187\uff0c\u6df7\u5300<\/li>\n<\/ul>\n<h4><span>\u86cb\u767d\u7535\u6cf3<\/span>Buffer<span>\uff08<\/span>5*<span>\uff09<\/span>1L<\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166834866.webp\" \/><\/p>\n<ul>\n<li>\u76f4\u63a5\u5b9a\u5bb9\u81f31L\uff0c\u65e0\u9700\u706d\u83cc\u548c\u8c03PH\uff1b\u4f7f\u7528\u524d\u7a00\u91ca\u81f31*<\/li>\n<\/ul>\n<h4>\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\uff08PBS\uff0c10*\uff09500ml<\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166864473.webp\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166869787.webp\" \/><\/p>\n<ul>\n<li>\u8c03\u5236PH=7.4-7.5<\/li>\n<\/ul>\n<h4>PBST<span>\u914d\u5236<\/span><\/h4>\n<ul>\n<li>1L\u7684PBS+5ml\u00b720%Tween20<\/li>\n<\/ul>\n<h4><span>\u5c01\u95ed\u6db2\uff08<\/span>5%<span>\uff09<\/span><\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166912488.webp\" \/><\/p>\n<ul>\n<li>\u5976\u7c895g\uff0c\u6eb6\u4e8ePBS\uff0c\u5b9a\u5bb9\u81f31L\u3002<\/li>\n<li>\u6ce8\uff1a\u63a8\u8350\u4f7f\u7528\u7684\u5976\u7c89\uff1a\u96c0\u5de2\u9ad8\u9499\u5976\u7c89\u6216\u591a\u529b\u7cbe\u4f4e\u8102\u5976\u7c89\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><span>\u4e00\u6297\u6eb6\u4e8e\u5c01\u95ed\u6db2<\/span><\/h4>\n<h4><span>\u4e8c\u6297\u6eb6\u4e8e<\/span>PBST<\/h4>\n<h4><span>\u914d\u5236<\/span>SDS-PAGE<span>\u7684\u6d53\u7f29\u80f6<\/span><\/h4>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166940241.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166946773.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166954170.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166961928.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166968967.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166976088.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677166990363.webp\" \/><\/p>\n<ul>\n<li>\u4e5f\u79f0\u5806\u79ef\u80f6\u3001\u79ef\u5c42\u80f6\u3001\u4e0a\u5c42\u80f6<\/li>\n<\/ul>\n<h4>\u9009\u62e9\u5408\u9002\u7684\u5206\u79bb\u80f6\uff08\u4e0b\u5c42\u80f6\uff09\u6d53\u5ea6<\/h4>\n<ul>\n<li>\u6839\u636e\u76ee\u7684\u86cb\u767d\u7684\u5206\u5b50\u91cf\u5927\u5c0f\u9009\u62e9<\/li>\n<\/ul>\n<h4><span><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677167006984.webp\" \/><\/span><\/h4>\n<h4><span>\u4e19\u70ef\u9170\u80fa<\/span><\/h4>\n<ul>\n<li>\u4e0e\u4e3a\u86cb\u767d\u8d28\u7535\u6cf3\u63d0\u4f9b\u8f7d\u4f53\uff0c\u5176\u51dd\u56fa\u7684\u597d\u574f\u76f4\u63a5\u5173\u7cfb\u5230\u7535\u6cf3\u6210\u529f\u4e0e\u5426\uff0c\u4e0e\u4fc3\u51dd\u5242\u53ca\u73af\u5883\u5bc6\u5207\u76f8\u5173<\/li>\n<li>\u6709\u6bd2\uff0c\u8fdb\u5165\u4eba\u4f53\u540e\uff0c\u4f1a\u5728\u4f53\u5185\u4e0eDNA\u4e0a\u7684\u9e1f\u560c\u5464\u7ed3\u5408\u5f62\u6210\u590d\u5408\u7269\uff0c\u5bfc\u81f4\u9057\u4f20\u7269\u8d28\u635f\u4f24\u548c\u57fa\u56e0\u7a81\u53d8\u3002<\/li>\n<\/ul>\n<h4>\u03b2-<span>\u5def\u57fa\u4e59\u9187<\/span><\/h4>\n<ul>\n<li>\u4f5c\u4e3a\u8fd8\u539f\u5242\u6dfb\u52a0\u5165\u6837\u54c1\u4e2d\uff0c\u53ef\u4ee5\u8fd8\u539f\uff08\u5206\u89e3\uff09\u5b58\u5728\u4e8e\u86cb\u767d\u8d28\u7684\u80bd\u94fe\u4e0a\u534a\u80f1\u6c28\u9178\u6b8b\u57fa\u4e4b\u95f4\u7684\u4e8c\u786b\u952e(S-S\u952e)\u3002S-S\u952e\u4f7f\u86cb\u767d\u8d28\u6298\u53e0\u5f62\u6210\u4e8c\u7ea7\u7ed3\u6784\u3002\u56e0\u4e3a\u86cb\u767d\u7684\u6784\u8c61\u4f1a\u5e72\u6270\u7535\u6cf3\u7684\u8fc1\u79fb\u7387\u3002\u6240\u4ee5\uff0c\u5f53\u7535\u6cf3\u65f6\uff0c\u6d88\u9664\u86cb\u767d\u6784\u8c61\u5bf9\u8fc1\u79fb\u7387\u7684\u5e72\u6270\u975e\u5e38\u91cd\u8981\u3002\u03b2-\u5def\u57fa\u4e59\u9187\u8fd8\u539f\u86cb\u767d\u7684\u4e8c\u786b\u952e\u4e3a\u6e38\u79bb\u7684-SH\uff0c\u6837\u672c\u4e2d\u6240\u6709\u7684\u86cb\u767d\u90fd\u53d8\u6210\u7ebf\u6027\u7684\u4e00\u7ea7\u7ed3\u6784\uff0c\u4f7f\u5176\u7535\u6cf3\u8fc1\u79fb\u7387\u53ea\u4e0e\u86cb\u767d\u5206\u5b50\u91cf\u6709\u5173\u3002<\/li>\n<\/ul>\n<h4>\u5341\u4e8c\u70f7\u57fa\u786b\u9178\u94a0\uff08SDS\uff09<\/h4>\n<ul>\n<li>\u5c06SDS\u6df7\u5408\u5728\u6837\u54c1\u7f13\u51b2\u6db2\u4e2d\u3002\u86cb\u767d\u6837\u672c\u7528\u03b2-\u5def\u57fa\u4e59\u9187\u5904\u7406\u540e\u89e3\u805a\u6210\u5355\u4e00\u7ebf\u6027\uff1b\u7528SDS\u5904\u7406\u540e\uff0c\u6240\u6709\u86cb\u767d\u8d28\u5747\u53ea\u5e26\u8d1f\u7535\u8377\u3002SDS\u53ef\u4ee5\u4f7f\u6240\u6709\u7684\u86cb\u767d\u8d28\u5206\u5b50\u5747\u5300\u7684\u5e26\u4e0a\u8d1f\u7535\u8377<\/li>\n<li>\u5f53\u65bd\u52a0\u7535\u538b\u65f6\uff0c\u6240\u6709\u7684\u86cb\u767d\u8d28\u90fd\u5411\u51dd\u80f6\u7684\u6b63\u6781\u8fc1\u79fb\u3002\u56e0\u4e3a\uff0c\u4e0d\u540c\u5927\u5c0f\u7684\u86cb\u767d\u8d28\u5f97\u5230\u7684\u8d1f\u7535\u8377\u4e0e\u5176\u5206\u5b50\u91cf\u6210\u6b63\u6bd4\uff0c\u5b83\u4eec\u53ea\u56e0\u4e3a\u5206\u5b50\u91cf\u5927\u5c0f\u7684\u4e0d\u540c\u800c\u5206\u79bb\u5f00\uff0c\u800c\u4e0d\u662f\u56e0\u4e3a\u5e26\u7535\u8377\u7684\u591a\u5c11\u6216\u8005\u86cb\u767d\u6784\u8c61\u3002\u5728\u51dd\u80f6\u4e2d\u4e5f\u5b58\u5728SDS\uff0c\u8fd9\u662f\u4e3a\u4e86\u786e\u4fdd\u6240\u6709\u7684\u86cb\u767d\u8d28\u5728\u6574\u4e2a\u51dd\u80f6\u4e2d\u4fdd\u6301\u8377\u8f7d\u8d1f\u7535\u8377\u3002<\/li>\n<li>\u4f5c\u4e3a\u9633\u79bb\u5b50\u53bb\u6c61\u5242\uff0c\u4f5c\u7528\u6709\u56db\uff1a\u53bb\u86cb\u767d\u8d28\u7535\u8377\u3001\u89e3\u79bb\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u6c22\u952e\u3001\u53d6\u6d88\u86cb\u767d\u5206\u5b50\u5185\u7684\u758f\u6c34\u4f5c\u7528\u3001\u53bb\u591a\u80bd\u6298\u53e0\u3002<\/li>\n<li>\u5bf9\u7c98\u819c\u548c\u4e0a\u547c\u5438\u9053\u6709\u523a\u6fc0\u4f5c\u7528\uff0c\u5bf9\u773c\u548c\u76ae\u80a4\u6709\u523a\u6fc0\u4f5c\u7528\uff0c\u53ef\u5f15\u8d77\u547c\u5438\u7cfb\u7edf\u8fc7\u654f\u6027\u53cd\u5e94\u3002<\/li>\n<\/ul>\n<h4><span>\u8fc7\u786b\u9178\u94f5\uff08<\/span>APS<span>\uff09<\/span><\/h4>\n<ul>\n<li>\u8fc7\u786b\u9178\u94f5\u662f\u63d0\u4f9b\u5f62\u6210\u81ea\u7531\u57fa\u7684\u786b\u9178\u76d0\u57fa\u56e2\u7684\u3002\u8fd9\u4e9b\u7528\u4e8e\u81ea\u7531\u57fa\u751f\u6210\u7684\u786b\u9178\u76d0\u7531\u8fc7\u786b\u9178\u94f5\u63d0\u4f9b<\/li>\n<li>\u5bf9\u9ecf\u819c\u3001\u4e0a\u547c\u5438\u9053\u7ec4\u7ec7\u3001\u773c\u775b\u548c\u76ae\u80a4\u5177\u6709\u6781\u5927\u7684\u5371\u5bb3\u6027\u3002\u773c\u3001\u76ae\u80a4\u63a5\u89e6\u53ef\u5f15\u8d77\u5f3a\u70c8\u523a\u6fc0\u3001\u75bc\u75db\u751a\u81f3\u707c\u4f24\uff0c\u5438\u5165\u53ef\u81f4\u547d\u3002<\/li>\n<\/ul>\n<h4>TEMED\u00b7(N,\u00b7N,\u00b7N',\u00b7N'-tetramethylethylenediamine)<\/h4>\n<ul>\n<li>\u51dd\u80f6\u7684\u805a\u5408\u662f\u901a\u8fc7\u81ea\u7531\u57fa\u673a\u5236\u53d1\u751f\u7684\u3002TEMED(N\uff0cN\uff0cN\uff0cN-\u56db\u7532\u57fa\u4e59\u4e8c\u80fa)\u4f5c\u4e3a\u50ac\u5316\u5242\uff0c\u751f\u6210\u786b\u9178\u76d0\u81ea\u7531\u57fa\u3002<\/li>\n<li>\u5177\u6709\u8150\u8680\u6027\u3001\u6325\u53d1\u6027\u3001\u5f3a\u795e\u7ecf\u6bd2\u6027\u3002<\/li>\n<\/ul>\n<h2><span>\u64cd\u4f5c\u6b65\u9aa4<\/span><\/h2>\n<h3><span>\u86cb\u767d\u6837\u54c1\u5236\u5907<\/span><\/h3>\n<h4><span>\u7ec4\u7ec7\u4e2d\u603b\u86cb\u767d\u7684\u63d0\u53d6<\/span><\/h4>\n<h4><span>\u7ec4\u7ec7\u86cb\u767d\u83b7\u53d6<\/span><\/h4>\n<ul>\n<li><span>\u6572\u53d6<\/span>0.07-0.1g<span>\u7ec4\u7ec7<\/span>+400-500ul<span>\u88c2\u89e3\u6db2<\/span><\/li>\n<li><span>\u653e\u5165\u6253\u6837\u673a\u6253\u6837<\/span><\/li>\n<li><span>\u5b9e\u9a8c\u5ba4\u7814\u94b5\u7814\u78e8<\/span><\/li>\n<li>\u53d6\u7a0d\u5927\u4e8e0.1g\u7684\u6837\u54c1\u653e\u5165\u7814\u94b5\u4e2d\uff0c\u6765\u56de\u78e8\uff0c\u81f3\u7c89\u672b\u72b6\uff0c\u7528\u67aa\u5934\u522e\u53d6\u6536\u96c6\u5165\u79bb\u5fc3\u7ba1<\/li>\n<\/ul>\n<h4><span>\u7ec6\u80de<\/span><\/h4>\n<ul>\n<li><span>\u53bb\u9664\u4e0a\u6e05\u6db2<\/span><\/li>\n<li><span>\u51b7<\/span>PBS<span>\u6d17<\/span>2<span>\u6b21<\/span><\/li>\n<li>0.25%\u7684\u80f0\u9176\u6d88\u5316\u540e\u8f6c\u79fb\u81f3EP\u7ba1\u4e2d\u79bb\u5fc3<\/li>\n<li><span>\u51b7<\/span>PBS<span>\u6d17<\/span>2<span>\u6b21<\/span><\/li>\n<li>\u52a0\u5165100\uff5e300\u00b7\u00b5L\u542b\u6709\u86cb\u767d\u9176\u6291\u5236\u5242\u7684\u7ec6\u80de\u88c2\u89e3\u6db2<\/li>\n<li>\u88c2\u89e330min\uff0c\u6bcf10min\u5439\u6253\u4e00\u6b21<\/li>\n<li><span>\u79bb\u5fc3\uff0c\u53d6\u86cb\u767d\u4e0a\u6e05<\/span><\/li>\n<\/ul>\n<h4><span>\u53d6\u51fa\u7684\u6837\u54c1\u653e\u7f6e\u51b0\u4e0a<\/span>1-2h<\/h4>\n<ul>\n<li>\uff08\u4e2d\u95f4\u6df7\u53005-6\u6b21\uff09\u76f4\u81f3\u88c2\u89e3\u5b8c\u5168<\/li>\n<\/ul>\n<h4><span>\u6837\u54c1\u79bb\u5fc3<\/span>*2<\/h4>\n<ul>\n<li>\u79bb\u5fc3\u6761\u4ef6\uff1a13000rpm\uff0c4\u2103\uff0c30min<\/li>\n<li>\u79bb\u5fc3\u673a\u63d0\u524d\u9884\u51b7\u81f34\u2103<\/li>\n<\/ul>\n<h4><span>\u53d6\u4e0a\u6e05\u4e8e\u65b0\u7684<\/span>EP<span>\u7ba1\u4e2d<\/span><\/h4>\n<h4><span>\u53ea\u8c08\u7ecf\u9a8c<\/span><\/h4>\n<ul>\n<li>\u597d\u7684\u86cb\u767d\u662fWB\u6210\u529f\u7684\u4e00\u534a<\/li>\n<li>\u5feb\u901f\u5f7b\u5e95\u88c2\u89e3\uff0c\u5148\u53d8\u6027\u540e\u4fdd\u5b58<\/li>\n<li>\u5c3d\u91cf\u9009\u62e9\u8db3\u591f\u5f3a\u7684\u88c2\u89e3\u6db2\uff08\u5982RIPA\u88c2\u89e3\u6db2\uff09<\/li>\n<li>\u5408\u9002\u7684\u76d0\u6d53\u5ea6\u4e0b\uff0c\u4fdd\u6301\u86cb\u767d\u8d28\u7684\u6700\u5927\u6eb6\u89e3\u6027\u548c\u53ef\u91cd\u590d\u6027<\/li>\n<li>\u5c3d\u91cf\u9664\u53bb\u6838\u9178\uff0c\u591a\u7cd6\uff0c\u8102\u7c7b\u7b49\u5e72\u6270\u5206\u5b50<\/li>\n<li>\u63d0\u53d6\u86cb\u767d\u8d28\u8fc7\u7a0b\u4e2d\uff0c\u5e94\u5728\u4f4e\u6e29\u73af\u5883\u4e0b\u8fdb\u884c\uff0c\u4ee5\u6291\u5236\u86cb\u767d\u9176\u7684\u6c34\u89e3\u4f5c\u7528\uff08\u53ef\u52a0\u5165\u9002\u5408\u7684\u86cb\u767d\u9176\u6291\u5236\u5242\uff09<\/li>\n<li>\u86cb\u767d\u6837\u54c1\u5efa\u8bae\u5206\u88c5\u540e\u51b7\u51bb\u5e72\u71e5\u6216\u76f4\u63a5\u4ee5\u6db2\u4f53\u6001\u7f6e-80\u2103\u4e2d\u4fdd\u5b58\uff0c\u4e0d\u8981\u53cd\u590d\u51bb\u878d<\/li>\n<li>\u86cb\u767d\u6d53\u5ea6\u4f4e\u65f6\uff0c\u53ef\u4f7f\u7528\u8d85\u6ee4\u819c\u6d53\u7f29\u6216\u8005\u7528\u771f\u7a7a\u51bb\u5e72\u673a\u5c06\u86cb\u767d\u51bb\u5e72<\/li>\n<\/ul>\n<h3><span>\u86cb\u767d\u542b\u91cf\u7684\u6d4b\u5b9a<\/span><\/h3>\n<p><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677168535985.webp\" \/><\/p>\n<ul>\n<li>\u786e\u4fdd\u6bcf\u4e2a\u86cb\u767d\u6837\u54c1\u7684\u4e0a\u6837\u91cf\u4e00\u81f4<\/li>\n<\/ul>\n<h4>96<span>\u5b54\u677f\u7b2c\u4e00\u5217<\/span>1-8<\/h4>\n<ul>\n<li>\u6d53\u5ea6\u4e3a0,1,2,4,6,8,9,10\u500d\u7684\u86cb\u767d\u6807\u51c6\u6db2+\u6c34=10\u621620ml<\/li>\n<li>\u52a0\u4e0a5\u500d\u7a00\u91ca\u7684\u8003\u9a6c\u65af\u4eae\u84dd\u67d3\u6db2200ml<\/li>\n<\/ul>\n<h4>96<span>\u5b54\u677f\u540e\u9762\u51e0\u5217<\/span><\/h4>\n<ul>\n<li>\u4f9d\u6b21\u52a0\u5165\u7a00\u91ca\u7684\u86cb\u767d\u6837\u54c1\u6216\u539f\u6db210-20ml<\/li>\n<li>5\u500d\u7a00\u91ca\u7684\u8003\u9a6c\u65af\u4eae\u84dd\u67d3\u6db2200ml<\/li>\n<\/ul>\n<h4>\u786e\u4fdd\u6bcf\u4e2a\u86cb\u767d\u6837\u54c1\u7684\u4e0a\u6837\u91cf\u4e00\u81f4<\/h4>\n<h3>SDS-PAGE<span>\u7535\u6cf3<\/span><\/h3>\n<h4><span>\u6982\u5ff5<\/span><\/h4>\n<ul>\n<li>\u5341\u4e8c\u70f7\u57fa\u786b\u9178\u94a0\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff08\u82f1\u8bed\uff1asodium\u00b7dodecyl\u00b7sulfate\u00b7polyacrylamide\u00b7gel\u00b7electrophoresis\uff0c\u7b80\u79f0SDS-PAGE\uff09\u662f\u80f6\u4f53\u7535\u6cf3\u7684\u4e00\u79cd\uff0c\u5e38\u7528\u4e8e\u751f\u7269\u5316\u5b66\u3001\u9274\u8bc6\u79d1\u5b66\u3001\u9057\u4f20\u5b66\u548c\u5206\u5b50\u751f\u7269\u5b66\u7b49\u9886\u57df\u7684\u5206\u6790\u6280\u672f\uff0c\u6b64\u9879\u6280\u672f\u7684\u539f\u7406\uff0c\u662f\u5229\u7528\u86cb\u767d\u8d28\u5206\u5b50\u91cf\u5927\u5c0f\u7684\u4e0d\u540c\uff0c\u4f7f\u5176\u5728\u7535\u6cf3\u80f6\u4e2d\u5206\u79bb\uff0c\u662f\u6700\u5e38\u7528\u7684\u86cb\u767d\u8d28\u5206\u6790\u6280\u672f\u4e4b\u4e00<\/li>\n<li>\u5728SDS-PAGE\u4e2d\u4f7f\u7528\u7684\u51dd\u80f6\u662f\u805a\u4e19\u70ef\u9170\u80fa\uff08polyacrylamide\uff09\uff0c\u4f7f\u86cb\u767d\u8d28\u5448\u7ebf\u6027\u5316\u7684\u8bd5\u5242\u662fSDS\u3002\u56e0\u6b64\u5f97\u540dSDS-PAGE<\/li>\n<\/ul>\n<h4><span>\u539f\u7406<\/span><\/h4>\n<h4><span>\u5237\u677f<\/span><\/h4>\n<h4><span>\u9a8c\u6f0f<\/span><\/h4>\n<ul>\n<li><span>\u5f85\u7535\u6cf3\u69fd\u5b89\u88c5\u5b8c\u6bd5<\/span><\/li>\n<li><span>\u7f13\u6162\u6ce8\u5165\u5c11\u91cf\u84b8\u998f\u6c34<\/span><\/li>\n<li>15s\u5185\u6ca1\u6709\u6f0f\u6db2\u5012\u6389\u84b8\u998f\u6c34<\/li>\n<li>\u5438\u6c34\u7eb8\u5c06\u6b8b\u7559\u7684\u6c34\u5438\u5e72\u51c0<\/li>\n<\/ul>\n<h4><span>\u914d\u80f6<\/span><\/h4>\n<p><strong>\u88c5\u677f<\/strong><\/p>\n<ul>\n<li>\u73bb\u7483\u677f\u5bf9\u9f50\u540e\u653e\u5165\u5939\u4e2d\u5361\u7d27<\/li>\n<li>\u5782\u76f4\u5361\u5728\u67b6\u5b50\u4e0a\u51c6\u5907\u704c\u80f6<\/li>\n<\/ul>\n<p><strong>\u914d\u5206\u79bb\u80f6<\/strong><\/p>\n<ul>\n<li><span>\u52a0\u5165<\/span>TEMED<span>\u540e\u7acb\u5373\u6447\u5300<\/span><\/li>\n<li>\u704c\u80f6\u81f3\u80f6\u9762\u5347\u5230\u73bb\u7483\u677f3\/4\u4f4d\u7f6e<\/li>\n<li><span>\u80f6\u4e0a\u52a0\u4e00\u5c42\u6c34<\/span><\/li>\n<li>\u6db2\u5c01\u540e\u7684\u80f6\u51dd\u7684\u66f4\u5feb<\/li>\n<li>\u52a0\u6c34\u6db2\u5c01\u65f6\u8981\u5f88\u6162\uff0c\u5426\u5219\u80f6\u4f1a\u88ab\u51b2\u53d8\u578b<\/li>\n<li>\u7b49\u6c34\u548c\u80f6\u4e4b\u95f4\u6709\u4e00\u6761\u6298\u5c04\u7ebf\u8bf4\u660e\u80f6\u5df2\u51dd\u4e86<\/li>\n<\/ul>\n<ul>\n<li>\u5012\u53bb\u80f6\u4e0a\u5c42\u6c34\u5e76\u7528\u5438\u6c34\u7eb8\u5c06\u6c34\u5438\u5e72<\/li>\n<\/ul>\n<p><strong>\u914d\u6d53\u7f29\u80f6<\/strong><\/p>\n<ul>\n<li><span>\u52a0\u5165<\/span>TEMED<span>\u540e\u7acb\u5373\u6447\u5300<\/span><\/li>\n<li><span>\u5c06\u5269\u4f59\u7a7a\u95f4\u704c\u6ee1\u6d53\u7f29\u80f6<\/span><\/li>\n<li><span>\u68b3\u5b50\u63d2\u5165\u6d53\u7f29\u80f6\u4e2d<\/span><\/li>\n<li>\u63d2\u68b3\u5b50\u65f6\u8981\u4f7f\u68b3\u5b50\u4fdd\u6301\u6c34\u5e73<\/li>\n<li><span>\u7b49\u6d53\u7f29\u80f6\u51dd\u56fa<\/span><\/li>\n<li>\u4e24\u624b\u5206\u522b\u634f\u4f4f\u68b3\u5b50\u7684\u4e24\u8fb9\u7ad6\u76f4\u5411\u4e0a\u8f7b\u8f7b\u62d4\u51fa<\/li>\n<li><span>\u6c34\u51b2\u6d17\u6d53\u7f29\u80f6<\/span><\/li>\n<\/ul>\n<p><strong>\u88c5\u7535\u6cf3\u69fd<\/strong><\/p>\n<ul>\n<li><span>\u5c0f\u73bb\u7483\u677f\u9762\u5411\u5185<\/span><\/li>\n<li>\u82e5\u53ea\u8dd1\u4e00\u5757\u80f6\uff0c\u69fd\u53e6\u4e00\u8fb9\u8981\u57ab\u4e00\u5757\u5851\u6599\u677f\u4e14\u6709\u5b57\u7684\u4e00\u9762\u9762\u5411\u5916<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><span>\u4e0a\u6837<\/span><\/h4>\n<ul>\n<li>\u8ba1\u7b97\u542b50mg\u86cb\u767d\u7684\u6eb6\u6db2\u4f53\u79ef(\u4e0a\u6837\u91cf)<\/li>\n<li><span>\u9002\u91cf\u86cb\u767d\u52a0\u5165<\/span>4\uff1a1\u00b7\u00b75*Loading\u00b7Buffer<span>\u4e8e\u79bb\u5fc3\u7ba1<\/span><\/li>\n<li>100\u2103<span>\u6c34\u4e2d\u716e<\/span>5min<span>\u4f7f\u86cb\u767d\u53d8\u6027<\/span><\/li>\n<li><span>\u653e\u7f6e\u51b0\u4e0a<\/span>3min<\/li>\n<li>\u79bb\u5fc315min\uff0c13000rpm\uff0c4\u2103<\/li>\n<li>\u52a0\u8db3\u591f\u76841*\u7535\u6cf3\u7f13\u51b2\u6db2\u540e\u5f00\u59cb\u51c6\u5907\u4e0a\u6837<\/li>\n<li>\u7535\u6cf3\u6db2\u81f3\u5c11\u8981\u6f2b\u8fc7\u5185\u6d4b\u7684\u5c0f\u73bb\u7483\u677f<\/li>\n<li>\u7528\u5fae\u91cf\u8fdb\u6837\u5668\u8d34\u58c1\u5438\u53d6\u6837\u54c1<\/li>\n<li>\u4e0d\u8981\u5438\u8fdb\u6c14\u6ce1<\/li>\n<li>\u5c06\u52a0\u6837\u5668\u9488\u5934\u63d2\u81f3\u52a0\u6837\u5b54\u4e2d\u7f13\u6162\u52a0\u5165\u6837\u54c1<\/li>\n<li>\u52a0\u6837\u592a\u5feb\u53ef\u4f7f\u6837\u54c1\u51b2\u51fa\u52a0\u6837\u5b54\uff0c\u82e5\u6709\u6c14\u6ce1\u4e5f\u53ef\u80fd\u4f7f\u6837\u54c1\u6ea2\u51fa<\/li>\n<\/ul>\n<h4><span>\u7535\u6cf3<\/span><\/h4>\n<ul>\n<li><span>\u6d53\u7f29\u80f6<\/span>80V<span>\u7535\u538b\u8dd1<\/span>20<span>\u5206\u949f<\/span><\/li>\n<li><span>\u5206\u79bb\u80f6<\/span>180V<span>\u7535\u538b\u8dd1\u81f3\u5e95\u7aef<\/span><\/li>\n<li><span>\u505c\u6b62\u7535\u6cf3<\/span><\/li>\n<\/ul>\n<h4><span>\u6e05\u6d17\u56de\u6536\u7269\u54c1<\/span><\/h4>\n<ul>\n<li><span>\u7eaf\u6c34\u51b2\u6d17\u73bb\u7483\u677f<\/span><\/li>\n<li><span>\u53d6\u80f6\uff0c\u6e05\u6d17\u5207\u53bb\u6d53\u7f29\u80f6<\/span><\/li>\n<li><span>\u5728<\/span>Marker<span>\u4e0b\u65b9\u5207\u89d2\u505a\u6807\u8bb0<\/span><\/li>\n<li><span>\u5c06\u80f6\u6ce1\u5728\u6e05\u6c34\u4e2d<\/span><\/li>\n<\/ul>\n<h4><span>\u53ea\u8c08\u7ecf\u9a8c<\/span><\/h4>\n<ul>\n<li><strong>\u80f6\u6700\u597d\u73b0\u914d\u73b0\u7528<\/strong><\/li>\n<li>\u5982\u679c\u9700\u8981\u4fdd\u5b58\uff0c\u6700\u597d\u7528\u6e7f\u6da6\u7684\u4fdd\u9c9c\u819c\u5305\u597d\u5e76\u7f6e\u4e8e4\u2103\u51b0\u7bb1\u4e2d<\/li>\n<li><\/li>\n<li><strong>\u8bbe\u7f6e\u5185\u53c2<\/strong><\/li>\n<li>\u68c0\u6d4b\u6574\u4e2a\u5b9e\u9a8c\u7cfb\u7edf\u6216\u6821\u51c6\u5b9e\u9a8c\u7ed3\u679c<\/li>\n<li>\u4e00\u822c\u6307\u7531\u7ba1\u5bb6\u57fa\u56e0\u7f16\u7801\u8868\u8fbe\u7684\u86cb\u767d<\/li>\n<li><\/li>\n<li><strong>\u5185\u53c2\u9009\u62e9\u539f\u5219<\/strong><\/li>\n<li><span>\u8bbe\u7f6e\u5408\u9002\u6b63\u786e\u7684\u5bf9\u7167<\/span><\/li>\n<li>\u786e\u4fddwestern\u00b7blot\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u7279\u5f02\u6027<\/li>\n<li><\/li>\n<li><strong>\u9633\u6027\u5bf9\u7167<\/strong><\/li>\n<li>\u660e\u786e\u8868\u8fbe\u68c0\u6d4b\u86cb\u767d\u7684\u7ec4\u7ec7\u6216\u7ec6\u80de\uff0c\u7528\u4e8e\u68c0\u6d4b\u6297\u4f53\u7684\u5de5\u4f5c\u6548\u7387<\/li>\n<li><\/li>\n<li><strong>\u9634\u6027\u5bf9\u7167<\/strong><\/li>\n<li>\u660e\u786e\u4e0d\u8868\u8fbe\u68c0\u6d4b\u86cb\u767d\u7ec4\u7ec7\u6216\u7ec6\u80de\uff0c\u7528\u4e8e\u68c0\u6d4b\u6297\u4f53\u7684\u7279\u5f02\u6027<\/li>\n<li><\/li>\n<li><strong>\u4e8c\u6297\u5bf9\u7167<\/strong><\/li>\n<li>\u4e0d\u52a0\u4e00\u6297\uff0c\u7528\u4e8e\u68c0\u6d4b\u4e8c\u6297\u7684\u7279\u5f02\u6027<\/li>\n<li><\/li>\n<li><strong>\u5185\u53c2\u5bf9\u7167<\/strong><\/li>\n<li>\u68c0\u6d4b\u6807\u672c\u7684\u8d28\u91cf\u548c\u4e8c\u6297\u7cfb\u7edf<\/li>\n<li><\/li>\n<li><strong>\u7a7a\u767d\u5bf9\u7167<\/strong><\/li>\n<li>\u4e0d\u52a0\u4e00\u6297\u548c\u4e8c\u6297\uff1b\u7528\u4e8e\u68c0\u6d4b\u819c\u7684\u6027\u8d28\u548c\u5c01\u95ed\u7684\u6548\u679c<\/li>\n<\/ul>\n<h3><span>\u8f6c\u819c<\/span><\/h3>\n<ul>\n<li>\u8dd1\u80f6\u65f6\u51c6\u5907\u8f6c\u819c\u7528\u54c1,\u6700\u4e3b\u8981\u8d76\u6c14\u6ce1<\/li>\n<li>western\u00b7blot\u6210\u8d25\u7684\u5173\u952e<\/li>\n<\/ul>\n<h4>NC<span>\u819c<\/span><\/h4>\n<ul>\n<li>\u5728\u4f4e\u79bb\u5b50\u8f6c\u79fb\u7f13\u51b2\u6db2\u7684\u73af\u5883\u4e0b\uff0c\u5927\u591a\u6570\u5e26\u8d1f\u7535\u8377\u7684\u86cb\u767d\u8d28\u4f1a\u4e0eNC\u819c\u53d1\u751f\u758f\u6c34\u4f5c\u7528\u800c\u7ed3\u5408\u5728\u4e00\u8d77\uff0c\u5177\u6709\u5f88\u9ad8\u7684\u4eb2\u548c\u529b\u3002\u4e14\u6613\u4e8e\u5c01\u95ed\u975e\u7279\u5f02\u6027\u7ed3\u5408\u3002\u5728\u975e\u79bb\u5b50\u53bb\u6c61\u5242\u7684\u4f5c\u7528\u4e0b\uff0c\u7ed3\u5408\u7684\u86cb\u767d\u6613\u4e8e\u88ab\u6d17\u8131\u3002\u5f53\u7136\uff0c\u6839\u636e\u88ab\u8f6c\u79fb\u7684\u86cb\u767d\u5206\u5b50\u91cf\u5927\u5c0f\uff0c\u8981\u9009\u62e9\u4e0d\u540c\u5b54\u5f84\u7684NC\u819c\u3002<\/li>\n<\/ul>\n<h4>PVDF<span>\u819c<\/span><\/h4>\n<ul>\n<li>PVDF\u819c\u662f\u4e00\u79cd\u758f\u6c34\u6027\u805a\u5408\u7269\uff0c\u5728\u6c34\u6eb6\u6db2\u4e2d\u4e0d\u4f1a\u88ab\u6d78\u6e7f\uff0c\u4e3a\u4e86\u5728\u6c34\u6027\u7f13\u51b2\u6db2\u4e2d\u4f7f\u7528PVDF\u819c\uff0c\u9996\u5148\u9700\u5c06\u5176\u6d78\u6ce1\u572850%\uff08v\/v\uff09\u7684\u9187\u6eb6\u6db2\u4e2d\uff08\u7532\u9187\u3001\u4e59\u9187\u548c\u5f02\u4e19\u9187\uff09\uff0c\u6d78\u6ce1\u540e\u4f1a\u6d3b\u5316\u819c\u4e0a\u7684\u6b63\u70b9\u57fa\u56e2\uff0c\u4f7f\u5176\u66f4\u6613\u4e0e\u5e26\u8d1f\u7535\u8377\u7684\u86cb\u767d\u7ed3\u5408\u3002<\/li>\n<li>\u540c\u6837\uff0c\u6839\u636e\u88ab\u8f6c\u79fb\u7684\u86cb\u767d\u5206\u5b50\u91cf\u5927\u5c0f\uff0c\u8981\u9009\u62e9\u4e0d\u540c\u5b54\u5f84\u7684PVDF\u819c\u3002<\/li>\n<\/ul>\n<h4><span>\u526a<\/span>PVDF<span>\u819c<\/span><\/h4>\n<ul>\n<li>\u63d0\u524d\u5207\u4e2a\u89d2<\/li>\n<\/ul>\n<h4><span>\u7532\u9187\u4e2d\u6ce1<\/span>2-3min<\/h4>\n<h4>\u819c\u3001\u6d77\u7ef5\u3001\u6ee4\u7eb8\u4e00\u8d77\u6ce1\u51651*\u8f6c\u819c\u6db2\u4e2d<\/h4>\n<ul>\n<li>\u8f6c\u819c\u6db2\u53ef\u56de\u6536<\/li>\n<\/ul>\n<h4><span>\u653e<\/span>4\u2103<span>\u4fdd\u5b58<\/span><\/h4>\n<h4><span>\u88c5\u914d\u5939\u5b50<\/span><\/h4>\n<ul>\n<li>\u9ed1\u80f6\u767d\u819c<\/li>\n<li>\u534a\u5e72\u8f6c\u79fb\uff1a\u7eb8&gt;\u51dd\u80f6&gt;\u819c&gt;\u7eb8(\u5747\u7528\u8f6c\u79fb\u7f13\u51b2\u6db2\u6d78\u6e7f)\uff1b<\/li>\n<li>\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u00b7\u6e7f\u8f6c\u79fb\uff1a\u6d77\u7ef5&gt;\u7eb8&gt;\u51dd\u80f6&gt;\u819c&gt;\u7eb8&gt;\u6d77\u7ef5(\u5168\u7a0b\u5728\u8f6c\u79fb\u7f13\u51b2\u6db2\u4e2d\u6d78\u6ce1)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\u5939\u5b50\u6253\u5f00\u4f7f\u9ed1\u7684\u4e00\u9762\u4fdd\u6301\u6c34\u5e73<\/li>\n<li>\u4e0a\u9762\u57ab\u4e00\u5f20\u6d77\u7ef5\u57ab\uff0c\u73bb\u68d2\u64c0\u53bb\u6c14\u6ce1<\/li>\n<li>\u53e6\u4e00\u624b\u8981\u538b\u4f4f\u57ab\u5b50\u4f7f\u5176\u4e0d\u80fd\u968f\u4fbf\u79fb\u52a8<\/li>\n<li>\u57ab\u5b50\u4e0a\u57ab2\u5c42\u6ee4\u7eb8\uff0c\u73bb\u68d2\u64c0\u53bb\u6c14\u6ce1<\/li>\n<li>\u80f6\u76d6\u4e8e\u6ee4\u7eb8\u4e0a\uff0c\u73bb\u68d2\u64c0\u53bb\u6c14\u6ce1<\/li>\n<li>\u5c06\u819c\u7528\u954a\u5b50\u76d6\u4e8e\u80f6\u4e0a\uff0c\u5e76\u9664\u6c14\u6ce1<\/li>\n<li>\u819c\u4e0a\u76d62\u5c42\u6ee4\u7eb8\u5e76\u9664\u53bb\u6c14\u6ce1<\/li>\n<li><span>\u76d6\u4e0a\u53e6\u4e00\u4e2a\u6d77\u7ef5\u57ab<\/span><\/li>\n<li><span>\u5408\u8d77\u5939\u5b50<\/span><\/li>\n<\/ul>\n<h4><span>\u88c5\u914d\u8f6c\u819c\u69fd<\/span><\/h4>\n<ul>\n<li><strong>\u5939\u5b50\u653e\u5165\u8f6c\u819c\u69fd<\/strong><\/li>\n<li>\u9ed1\u5bf9\u9ed1,\u7ea2\u5bf9\u7ea2<\/li>\n<li><\/li>\n<li><strong>\u8f6c\u819c\u69fd\u4e2d\u653e\u5165\u51b0\u76d2<\/strong><\/li>\n<li>\u7535\u8f6c\u79fb\u65f6\u4f1a\u4ea7\u70ed\uff0c\u8fc7\u70ed\u6709\u53ef\u80fd\u9020\u6210\u86cb\u767d\u8d28\u7684\u964d\u89e3\uff0c\u540c\u65f6\u5927\u91cf\u4ea7\u70ed\uff0c\u4f1a\u4f7f\u51dd\u80f6\u81a8\u80c0\uff0c\u6709\u53ef\u80fd\u5728\u80f6\u548c\u819c\u4e4b\u95f4\u4ea7\u751f\u7a7a\u9699\uff0c\u5f15\u8d77\u8f6c\u819c\u4e0d\u5747\u5300\uff0c\u6240\u4ee5\u5728\u8f6c\u819c\u69fd\u4e2d\u653e\u5165\u51b0\u76d2\uff0c\u5728\u51b0\u6c34\u6df7\u5408\u7269\u4e2d\u8fdb\u884c\u8f6c\u819c<\/li>\n<li><\/li>\n<li><strong>\u8f6c\u819c\u6761\u4ef6<\/strong><\/li>\n<li><span>\u7535\u6d41<\/span>300mA<span>\u6216\u7535\u538b<\/span>70V<span>\uff0c<\/span>2h<\/li>\n<li>\u7535\u538b\u5fc5\u987b\u63a7\u5236\u572860-70V\uff0c\u8c03\u8282\u7535\u6d41<\/li>\n<\/ul>\n<h4><span>\u8f6c\u819c<\/span><\/h4>\n<h4><span>\u6e05\u6d17\u56de\u6536\u7269\u54c1<\/span><\/h4>\n<ul>\n<li>\u819c\u653e\u5165\u88c5\u6c34\u7684\u5c0f\u76d2\u4e2d\u51b2\u6d17<\/li>\n<li><span>\u52a0<\/span>5%<span>\u7684\u5c01\u95ed\u6db2<\/span><\/li>\n<li><span>\u6447\u5e8a\u5c01\u95ed<\/span>1h<\/li>\n<\/ul>\n<h4><span>\u53ea\u8c08\u7ecf\u9a8c<\/span><\/h4>\n<ul>\n<li><span>\u80f6\u5728\u8d1f\u6781\uff0c\u819c\u9760\u8fd1\u6b63\u6781<\/span><\/li>\n<li>\u6ee4\u7eb8\u4e0d\u8981\u5927\u8fc7\u819c\uff0c\u9632\u6b62\u77ed\u8def<\/li>\n<li>\u5939\u597d\u819c\u548c\u51dd\u80f6\u540e\uff0c\u786e\u5b9a\u5728\u51dd\u80f6\u3001\u819c\u548c\u6ee4\u7eb8\u4e4b\u95f4\u6ca1\u6709\u6c14\u6ce1\u5b58\u5728\uff0c\u5426\u5219\u4f1a\u5bfc\u81f4\u8f6c\u819c\u4e0d\u5b8c\u5168<\/li>\n<li>\u4e00\u5b9a\u8981\u6234\u624b\u5957\u6216\u5851\u6599\u954a\u5b50\u63a5\u89e6\u819c\n<ul>\n<li>\u907f\u514d\u624b\u4e0a\u7684\u86cb\u767d\u548c\u6cb9\u8102\u964d\u4f4e\u8f6c\u819c\u6548\u7387<\/li>\n<\/ul>\n<\/li>\n<li><\/li>\n<li>\u6700\u597d\u628a\u8f6c\u819c\u69fd\u653e\u7f6e\u5728\u51b0\u6d74\u4e2d\u8fdb\u884c\u8f6c\u819c<\/li>\n<li><span>\u6e7f\u8f6c\u6cd5<\/span>\n<ul>\n<li>\u8f6c\u819c\u7684\u7535\u6d41\u5728200mA-400mA\u4e4b\u95f4<\/li>\n<li><span>\u8f6c\u819c\u65f6\u95f4\u4e3a<\/span>30-60<span>\u5206\u949f<\/span>\n<ul>\n<li>\u53ef\u4ee5\u572815-20mA\u8f6c\u819c\u8fc7\u591c<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<li><\/li>\n<li><span>\u5927\u7247\u6bb5\u7684<\/span>&gt;50KD<span>\u7684\u53ef\u4ee5\u9009\u7528<\/span>350mA,<span>\u5c0f\u7247\u6bb5\u7684\u53ef\u4ee5\u7528<\/span>250mA<\/li>\n<li>\u8f6c\u819c\u65f6\u95f4\u8981\u6839\u636e\u76ee\u7684\u86cb\u767d\u7684\u5927\u5c0f\u800c\u5b9a\n<ul>\n<li>\u76ee\u7684\u86cb\u767d\u7684\u5206\u5b50\u91cf\u8d8a\u5927\uff0c\u9700\u8981\u7684\u8f6c\u819c\u65f6\u95f4\u8d8a\u957f\uff0c\u76ee\u7684\u86cb\u767d\u7684\u5206\u5b50\u91cf\u8d8a\u5c0f\uff0c\u9700\u8981\u7684\u8f6c\u819c\u65f6\u95f4\u8d8a\u77ed<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span>\u6297\u4f53\u5b75\u80b2<\/span><\/h3>\n<p><span><img decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2023\/02\/1677167958452.webp\" \/><\/span><\/p>\n<h4><span>\u5b75\u4e00\u6297<\/span><\/h4>\n<p>\u53ea\u6709\u76ee\u7684\u86cb\u767d\u624d\u80fd\u4e0e\u4e00\u6297\u7ed3\u5408\u5f62\u6210\u6297\u539f\u6297\u4f53\u590d\u5408\u7269<\/p>\n<ul>\n<li><span>\u56de\u6536\u5c01\u95ed\u6db2<\/span><\/li>\n<li><span>\u52a0\u5165\u4e00\u6297<\/span><\/li>\n<li><span>\u6447\u5e8a<\/span>30min-1h<\/li>\n<li>4\u2103<span>\u8fc7\u591c<\/span><\/li>\n<li><span>\u5b75\u80b2<\/span>30min-1h<\/li>\n<li><span>\u4e00\u6297\u56de\u6536<\/span><\/li>\n<li>PBST\u6d17\uff0c\u6d175\u6b21\uff0c\u6bcf\u6b215min<\/li>\n<\/ul>\n<h4><span>\u5b75\u4e8c\u6297<\/span><\/h4>\n<p>\u4e8c\u6297\uff1a\u00a0\u00a0\u4e00\u6297\u7684\u6297\u4f53\uff0c\u5982\u4e00\u6297\u662f\u9f20\u4e00\u6297\uff0c\u5219\u4e8c\u6297\u5c31\u662f\u6297\u9f20IgG\u7684\u6297\u4f53<\/p>\n<p>\u5e26\u6709\u9176\uff08\u78b1\u6027\u78f7\u9178\u9176\u3001\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176\uff09\u6807\u8bb0\u7684\u4e8c\u6297\uff08\u4e5f\u662f\u4fd7\u79f0\u7684\u9176\u6807\u4e8c\u6297\uff09\u4e0e\u4e00\u6297\u7ed3\u5408\u5f62\u6210\u6297\u4f53\u590d\u5408\u7269\uff1b<\/p>\n<p>\u53ef\u7528\u6765\u6307\u793a\u4e00\u6297\u7684\u4f4d\u7f6e\uff0c\u4e5f\u5373\u6307\u793a\u4e86\u76ee\u7684\u86cb\u767d\u7684\u4f4d\u7f6e<\/p>\n<ul>\n<li><span>\u52a0\u4e8c\u6297<\/span><\/li>\n<li><span>\u5b75\u80b2<\/span>2h<\/li>\n<li><span>\u4e8c\u6297\u56de\u6536<\/span><\/li>\n<li>PBST\u6d17\uff0c\u6d175\u6b21\uff0c\u6bcf\u6b215min<\/li>\n<li><span>\u7eaf\u6c34\u51b2\u6d17<\/span>5-7<span>\u6b21<\/span><\/li>\n<li><span>\u5012\u6389\u7eaf\u6c34<\/span><\/li>\n<li><span>\u52a0\u5316\u5b66\u53d1\u5149\u6d78\u6ce1<\/span>2-3min<\/li>\n<\/ul>\n<h4><span>\u53ea\u8c08\u7ecf\u9a8c<\/span><\/h4>\n<ul>\n<li>\u5c01\u95ed\u6db2\u7684\u9009\u53d6\u5177\u4f53\u5f97\u770b\u6297\u4f53\u8bf4\u660e\u4e66<\/li>\n<li>\u5c01\u95ed\u6db2\u6d53\u5ea6\u7684\u9009\u62e9\u4e5f\u662f\u5177\u4f53\u5f97\u770b\u6297\u4f53\u7684\u8981\u6c42\n<ul>\n<li>\u4e00\u822c\u60c5\u51b55%BSA\u4f1a\u6bd45%\u725b\u5976\u7684\u6548\u679c\u597d<\/li>\n<\/ul>\n<\/li>\n<li><\/li>\n<li><span>\u6297\u4f53\u9009\u62e9<\/span><\/li>\n<\/ul>\n<h4><span>\u6210\u50cf<\/span><\/h4>\n<h4>WB<span>\u7ed3\u679c\u5b9a\u91cf\u5206\u6790<\/span><\/h4>\n<ul>\n<li>Quantiy\u00b7One<\/li>\n<li>ImageJ\n<ul>\n<li><span>\u7070\u5ea6\u5206\u6790<\/span><\/li>\n<li><span>\u5bc6\u5ea6\u5206\u6790<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3><span>\u5e38\u89c1\u95ee\u9898<\/span><\/h3>\n<h4><span>\u80f6\u4e0d\u5e73\u6216\u6f0f\u6db2<\/span><\/h4>\n<ul>\n<li><span>\u80f6\u677f\u6d17\u5237\u5e72\u51c0<\/span><\/li>\n<li>\u52a0\u5165AP\u548cTEMED\u7684\u91cf\u8981\u5408\u9002<\/li>\n<li>\u52a0\u5165\u8bd5\u5242\u540e\u6447\u5300\uff0c\u4f7f\u5176\u5145\u5206\u6df7\u5408\uff0c\u9632\u6b62\u90e8\u5206\u80f6\u5757\u805a\u5408\u4e0d\u5747\u5300<\/li>\n<li>\u6e29\u5ea6\u5408\u9002\uff0c\u53d7\u70ed\u4e0d\u5747\u5300\u5bfc\u81f4\u80f6\u805a\u5408\u4e0d\u5747\u5300<\/li>\n<li><span>\u4e24\u5757\u73bb\u7483\u677f\u5e95\u90e8\u8981\u5bf9\u9f50<\/span><\/li>\n<\/ul>\n<h4>\u6761\u5e26\u6bd4\u6b63\u5e38\u7684\u7a84\uff0c\u201c\u5fae\u7b11\u201d\u6216\u201c\u5012\u5fae\u7b11\u201d\u6761\u5e26<\/h4>\n<ul>\n<li><span>\u51dd\u80f6\u805a\u5408\u4e0d\u5747\u5300<\/span><\/li>\n<li><span>\u62d4\u68b3\u5b50\u8981\u8fc5\u901f<\/span><\/li>\n<li>\u6e05\u6d17\u52a0\u6837\u5b54\u8981\u5c0f\u5fc3\uff0c\u4ee5\u514d\u628a\u4e0a\u6837\u5e26\u626d\u66f2<\/li>\n<li><span>\u7eaf\u5316\u6837\u54c1\uff0c\u8c03\u6574\u76d0\u6d53\u5ea6<\/span>\n<ul>\n<li>\u6837\u54c1\u76d0\u6d53\u5ea6\u8fc7\u9ad8\u4f1a\u6324\u538b\u5176\u4ed6\u6761\u5e26\u5bfc\u81f4\u5bbd\u7a84\u4e0d\u4e00<\/li>\n<\/ul>\n<\/li>\n<li><\/li>\n<li><span>\u8d76\u8d70\u6c14\u6ce1<\/span>\n<ul>\n<li>\u80f6\u677f\u5e95\u90e8\u6709\u6c14\u6ce1\u4f1a\u5f71\u54cd\u7535\u6cf3\u6548\u679c<\/li>\n<\/ul>\n<\/li>\n<li><\/li>\n<li>\u6ce8\u610f\u7535\u6cf3\u69fd\u88c5\u7f6e\u662f\u5426\u5408\u9002<\/li>\n<\/ul>\n<h4><span>\u7535\u6cf3\u7684\u6761\u5e26\u5f88\u7c97<\/span><\/h4>\n<ul>\n<li><span>\u4e3b\u8981\u662f\u672a\u6d53\u7f29\u597d<\/span>\n<ul>\n<li><span>\u9002\u5f53\u589e\u52a0\u6d53\u7f29\u80f6\u7684\u957f\u5ea6<\/span><\/li>\n<li>\u4fdd\u8bc1\u6d53\u7f29\u80f6\u8d2e\u6db2\u7684pH\u6b63\u786e(6.7)<\/li>\n<li><span>\u9002\u5f53\u964d\u4f4e\u7535\u538b<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h4>\u51dd\u80f6\u80bf\u80c0\u6216\u5377\u66f2\uff0c\u6761\u5e26\u6b6a\u659c\u6216\u6f02\u79fb\uff0c\u5355\u4e2a\u6216\u591a\u4e2a\u767d\u70b9\uff0c\u8f6c\u819c\u7f13\u51b2\u6db2\u8fc7\u70ed<\/h4>\n<ul>\n<li>\u5c06\u51dd\u80f6\u5728\u8f6c\u819c\u4e4b\u524d\u653e\u5230\u8f6c\u819c\u7f13\u51b2\u6db2\u4e2d\u6d78\u6ce15-10min<\/li>\n<li>\u5728\u4e24\u5757\u6d77\u7ef5\u4e4b\u95f4\u57ab\u4e0a\u5c11\u8bb8\u666e\u901a\u7684\u8349\u7eb8<\/li>\n<li>\u786e\u4fdd\u819c\u548c\u80f6\u5757\u4e4b\u95f4\u6ca1\u6709\u6c14\u6ce1<\/li>\n<li><span>\u7f13\u51b2\u6db2\u4e2d\u79bb\u5b50\u6d53\u5ea6\u592a\u4f4e<\/span><\/li>\n<li><span>\u7535\u6d41\u6216\u7535\u538b\u592a\u9ad8<\/span><\/li>\n<\/ul>\n<h4><span>\u8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