{"id":564,"date":"2024-07-11T12:19:01","date_gmt":"2024-07-11T12:19:01","guid":{"rendered":"https:\/\/yunshangtulv.com.cn\/?p=564"},"modified":"2024-07-11T15:04:21","modified_gmt":"2024-07-11T15:04:21","slug":"%e5%ae%9e%e9%aa%8c%e5%b0%8f%e7%99%bd%e6%88%90%e9%95%bf%e8%b7%af%ef%bc%9a%e5%85%8b%e9%9a%86%e5%bd%a2%e6%88%90%e5%ae%9e%e9%aa%8c","status":"publish","type":"post","link":"https:\/\/yunshangtulv.com.cn\/?p=564","title":{"rendered":"\u5b9e\u9a8c\u5c0f\u767d\u6210\u957f\u8def\uff1a\u514b\u9686\u5f62\u6210\u5b9e\u9a8c"},"content":{"rendered":"

\u80cc\u666f\u77e5\u8bc6<\/p>

<\/div><\/div><\/h1>\n

\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\u662f\u7814\u7a76\u7ec6\u80de\u589e\u6b96\u80fd\u529b\u548c\u5b58\u6d3b\u7387\u7684\u6709\u6548\u65b9\u6cd5\u4e4b\u4e00\u3002\u8d34\u58c1\u540e\u7684\u7ec6\u80de\u4e0d\u4e00\u5b9a\u6bcf\u4e2a\u90fd\u80fd\u589e\u6b96\u548c\u5f62\u6210\u514b\u9686\uff0c\u800c\u5f62\u6210\u514b\u9686\u7684\u7ec6\u80de\u5fc5\u4e3a\u8d34\u58c1\u548c\u6709\u589e\u6b96\u6d3b\u529b\u7684\u7ec6\u80de\u3002<\/p>\n

\u901a\u8fc7\u89c2\u5bdf\u5355\u4e2a\u7ec6\u80de\u5728\u4f53\u5916\u57f9\u517b\u73af\u5883\u4e0b\u7684\u589e\u6b96\u548c\u514b\u9686\u5f62\u6210\u60c5\u51b5\uff0c\u53ef\u4ee5\u8bc4\u4f30\u7ec6\u80de\u7684\u589e\u6b96\u80fd\u529b\u548c\u7fa4\u4f53\u4f9d\u8d56\u6027\u3002<\/p>\n

\u8be5\u5b9e\u9a8c\u5305\u62ec\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\u548c\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\uff0c\u5206\u522b\u9002\u7528\u4e8e\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u3002<\/p>\n

\u00a0

\u57fa\u7840\u7406\u8bba\u77e5\u8bc6<\/p>

<\/div><\/div><\/span><\/h1>\n

<\/span>\u514b\u9686\u5f62\u6210<\/h2>\n

\u514b\u9686\u5f62\u6210\u662f\u6307\u5355\u4e2a\u7ec6\u80de\u5728\u4f53\u5916\u6301\u7eed\u5206\u88c2\u7e41\u6b96\u8d85\u8fc76<\/span>\u4ee3\u540e\uff0c\u5176\u540e\u4ee3\u7ec4\u6210\u7684\u7ec6\u80de\u7fa4\u3002\u5149\u955c\u4e0b\uff0c\u5927\u4e8e50<\/span>\u4e2a\u7ec6\u80de\uff0c\u8ba1\u6570\u4e3a1<\/span>\u514b\u9686\u3002\uff0c\u5927\u5c0f\u57280.3-1.0 mm<\/span>\u4e4b\u95f4\u3002<\/p>\n

\u514b\u9686\u5f62\u6210\u7387\u662f\u6307\u63a5\u79cd\u7ec6\u80de\u540e\uff0c\u6210\u6d3b\u5e76\u5f62\u6210\u514b\u9686\u7684\u7ec6\u80de\u6570\u5360\u603b\u63a5\u79cd\u7ec6\u80de\u6570\u7684\u767e\u5206\u6bd4\u3002\u53cd\u6620\u7ec6\u80de\u7fa4\u4f53\u4f9d\u8d56\u6027\u548c\u589e\u6b96\u80fd\u529b\u4e24\u4e2a\u91cd\u8981\u6027\u72b6\u3002<\/p>\n

\u589e\u6b96\u80fd\u529b<\/h2>\n

\u7ec6\u80de\u589e\u6b96\u80fd\u529b\u662f\u6307\u7ec6\u80de\u5728\u9002\u5b9c\u6761\u4ef6\u4e0b\u8fdb\u884c\u6709\u4e1d\u5206\u88c2\u7684\u80fd\u529b\u3002\u901a\u8fc7\u8ba1\u7b97\u514b\u9686\u5f62\u6210\u7387\uff0c\u53ef\u4ee5\u8bc4\u4f30\u7ec6\u80de\u7684\u589e\u6b96\u80fd\u529b\u3002<\/p>\n

\u00a0<\/span>\u7fa4\u4f53\u4f9d\u8d56\u6027<\/h2>\n

\u7fa4\u4f53\u4f9d\u8d56\u6027\u6307\u7684\u662f\u7ec6\u80de\u662f\u5426\u9700\u8981\u76f8\u4e92\u4f5c\u7528\u624d\u80fd\u5f62\u6210\u514b\u9686\u3002\u514b\u9686\u5f62\u6210\u7387\u4e5f\u80fd\u53cd\u6620\u7ec6\u80de\u7684\u72ec\u7acb\u751f\u5b58\u80fd\u529b\u548c\u7fa4\u4f53\u4f9d\u8d56\u6027\u3002<\/p>\n

\u5e94\u7528\u8303\u56f4<\/p>

<\/div><\/div><\/h1>\n

- <\/span>\u8bc4\u4f30\u7ec6\u80de\u589e\u6b96\u80fd\u529b<\/p>\n

- <\/span>\u7814\u7a76\u7ec6\u80de\u7fa4\u4f53\u4f9d\u8d56\u6027<\/p>\n

- <\/span>\u7b5b\u9009\u836f\u7269\u548c\u6cbb\u7597\u65b9\u6848\u7684\u6709\u6548\u6027<\/p>\n

- <\/span>\u8bc4\u4f30\u7ec6\u80de\u5728\u4f53\u5185\u5f62\u6210\u80bf\u7624\u7684\u6f5c\u529b\uff1a\u764c\u7ec6\u80de\u4e0d\u4e00\u5b9a\u90fd\u53ef\u4ee5\u5728\u4f53\u5185\u6210\u7624\uff0c\u4f46\u82e5\u4f53\u5916\u514b\u9686\u80fd\u529b\u8d8a\u5f3a\uff0c\u5373\u8868\u660e\u4f53\u5185\u6210\u7624\u6027\u8d8a\u5f3a\uff0c\u7b97\u662f\u6a21\u62df\u4f53\u5185\u6210\u7624\u7684\u4f53\u5916\u5b9e\u9a8c\u3002<\/p>\n

\u00a0

\u57fa\u672c\u539f\u7406<\/p>

<\/div><\/div><\/span><\/h1>\n

\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\uff08Colony Formation on Solid Media\uff09<\/h2>\n

\u9002\u7528\u4e8e\u8d34\u58c1\u751f\u957f\u7684\u7ec6\u80de\uff0c\u901a\u8fc7\u8ba1\u6570\u514b\u9686\u6570\u6765\u8bc4\u4f30\u7ec6\u80de\u7684\u589e\u6b96\u80fd\u529b\u548c\u7fa4\u4f53\u4f9d\u8d56\u6027\u3002<\/p>\n

\u00a0<\/span>\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\uff08Soft Agar Colony Formation\uff09<\/h2>\n

\u9002\u7528\u4e8e\u60ac\u6d6e\u751f\u957f\u7684\u7ec6\u80de\u548c\u80bf\u7624\u7ec6\u80de\uff0c\u901a\u8fc7\u5728\u743c\u8102\u4e2d\u5f62\u6210\u514b\u9686\u6765\u8bc4\u4f30\u7ec6\u80de\u7684\u589e\u6b96\u80fd\u529b\u548c\u6076\u6027\u7a0b\u5ea6\u3002\u6b63\u5e38\u7ec6\u80de\u4f9d\u9760\u7ec6\u80de\u4e0e\u7ec6\u80de\u5916\u57fa\u8d28\u7684\u63a5\u89e6\u624d\u80fd\u751f\u957f\u548c\u5206\u88c2\uff0c\u76f8\u53cd\uff0c\u4e0d\u7ba1\u5468\u56f4\u73af\u5883\u5982\u4f55\uff0c\u8f6c\u5316\u7ec6\u80de\u5747\u5177\u6709\u751f\u957f\u548c\u5206\u5316\u7684\u80fd\u529b\uff0c\u56e0\u6b64\u80fd\u591f\u4ee5\u951a\u5b9a\u72ec\u7acb\u7684\u65b9\u5f0f\u5f62\u6210\u83cc\u843d\u7684\u7ec6\u80de\u88ab\u8ba4\u4e3a\u662f\u8f6c\u5316\u548c\u81f4\u764c\u7684\u3002\u8fd9\u79cd\u65b9\u6cd5\u7684\u603b\u4f53\u76ee\u6807\u662f\u4ee5\u534a\u5b9a\u91cf\u548c\u4e25\u683c\u7684\u65b9\u5f0f\u6d4b\u5b9a\u7ec6\u80de\u7684\u8fd9\u79cd\u80fd\u529b\u3002<\/p>\n

\u8bbe\u5907\u8017\u6750<\/p>

<\/div><\/div><\/h1>\n

\u00a0<\/span>\u00a0<\/span>\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

- 0.25% <\/span>\u80f0\u9176\u6eb6\u6db2<\/p>\n

- <\/span>\u542b10% <\/span>\u80ce\u725b\u8840\u6e05\u7684\u57f9\u517b\u57fa<\/p>\n

- PBS <\/span>\u6eb6\u6db2<\/p>\n

- 4% <\/span>\u591a\u805a\u7532\u919b\u6eb6\u6db2<\/p>\n

- <\/span>\u7ed3\u6676\u7d2b\u67d3\u6db2\u6216\u59ec\u59c6\u8428\u67d3\u6db2\uff1a\u7ed3\u6676\u7d2b\u662f\u78b1\u6027\u67d3\u6599\uff0c\u53ef\u4ee5\u4e0e\u6838\u9178\u7ed3\u5408\u628a\u7ec6\u80de\u6838\u67d3\u6210\u84dd\u8272\uff0c\u4fbf\u4e8e\u89c2\u5bdf\u53ca\u8ba1\u6570<\/p>\n

- 6<\/span>\u5b54\u677f<\/p>\n

<\/span>\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

- 0.25% <\/span>\u80f0\u9176\u6eb6\u6db2<\/p>\n

- <\/span>\u542b10% <\/span>\u80ce\u725b\u8840\u6e05\u7684\u57f9\u517b\u57fa<\/p>\n

- PBS <\/span>\u6eb6\u6db2<\/p>\n

- <\/span>\u8d85\u7eaf\u6c34<\/p>\n

- <\/span>\u4f4e\u7194\u70b9\u743c\u8102\u7cd6<\/p>\n

- 2\u00d7<\/span>\u5b8c\u5168\u57f9\u517b\u57fa<\/p>\n

- 6<\/span>\u5b54\u677f<\/p>\n

\u5b9e\u9a8c\u51c6\u5907<\/p>

<\/div><\/div><\/span><\/h1>\n

\u00a0<\/span>\u00a0<\/span>\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

\u67d3\u6db2\u914d\u65b9<\/h3>\n

- 10\u00d7Giemsa\u67d3\u8272\u6db2<\/strong>\uff1aGiemsa<\/span>\u7c89\u672b0.8g <\/span>\u6eb6\u4e8e50mL<\/span>\u7532\u9187\uff0c\u5b8c\u5168\u6eb6\u89e3\u540e\u52a0\u516550mL<\/span>\u7518\u6cb9\uff0c\u6447\u5300\uff0c37\u2103-40\u2103<\/span>\u6c34\u6d748-12h<\/span>\uff0c\u68d5\u8272\u74f6\u5e38\u6e29\u4fdd\u5b58\uff0c\u7528\u65f6\u53d61<\/span>\u4efdGiemsa<\/span>\u539f\u6db2\u6eb6\u4e8e9<\/span>\u4efdPBS<\/span>\u4e2d\u3002<\/p>\n

- 0.1% \u7ed3\u6676\u7d2b\u67d3\u8272\u6db2\uff1a<\/strong>\u79f0\u53d61g<\/span>\u7ed3\u6676\u7d2b\u7c89\u672b\uff0c\u7528\u7532\u9187\u5b9a\u5bb9\u5230100mL<\/span>\uff0c\u907f\u5149\u5e38\u6e29\u4fdd\u5b58\u3002<\/p>\n

<\/span>\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

- <\/span>\u914d\u52361.2% <\/span>\u548c0.7%<\/span>\u743c\u8102\u7cd6\u6eb6\u6db2\uff0c\u9ad8\u538b\u706d\u83cc\uff0c\u7ef4\u6301\u572840\u2103<\/span>\u9632\u6b62\u51dd\u56fa\u3002<\/p>\n

\u64cd\u4f5c\u6b65\u9aa4<\/p>

<\/div><\/div><\/h1>\n

\u00a0<\/span>\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

\u00a0<\/span>\u5236\u5907\u7ec6\u80de\u60ac\u6db2<\/h3>\n

\u00a0 \u00a0- <\/span>\u5c06\u5bf9\u6570\u751f\u957f\u671f\u7684\u5355\u5c42\u7ec6\u80de\u5e38\u89c4\u6d88\u5316\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\uff0c\u91cd\u60ac\u7ec6\u80de\u6c89\u6dc0\uff0c\u8ba1\u6570\uff0c\u7a00\u91ca\u81f31\u00d710\u00b3<\/span>\u4e2a\/mL<\/span>\u3002<\/p>\n

\u00a0<\/span>\u63a5\u79cd\u7ec6\u80de<\/h3>\n

\u00a0\u00a0 - <\/span>\u4ee5\u6bcf\u5b5450<\/span>\u3001100<\/span>\u3001200<\/span>\u4e2a\u7ec6\u80de\u7684\u68af\u5ea6\u5bc6\u5ea6\u63a5\u79cd\u516d\u5b54\u677f\u4e2d\uff0c\u8865\u52a03mL<\/span>\u57f9\u517b\u57fa\uff0c\u7f6e\u4e8e37\u2103,5% CO<\/span>\u2082\u57f9\u517b\u7bb1\u4e2d\u57f9\u517b\uff0c\u9759\u7f6e\u57f9\u517b2-3<\/span>\u5468\u3002\u7ecf\u5e38\u89c2\u5bdf\uff0c\u5f53\u57f9\u517b\u76bf\u4e2d\u51fa\u73b0\u8089\u773c\u53ef\u89c1\u7684\u514b\u9686\u65f6\uff0c\u7ec8\u6b62\u57f9\u517b<\/p>\n

\u67d3\u8272<\/h3>\n

\u00a0\u00a0 - <\/span>\u5f53\u57f9\u517b\u76bf\u4e2d\u51fa\u73b0\u8089\u773c\u53ef\u89c1\u7684\u514b\u9686\u65f6\uff0c\u505c\u6b62\u57f9\u517b\uff0c\u5f03\u6389\u57f9\u517b\u57fa\uff0c\u7528PBS<\/span>\u5c0f\u5fc3\u6d17\u6da42-3<\/span>\u6b21\uff0c\u5f03\u6389PBS<\/span>\u3002\u6bcf\u5b54\u52a0\u51654%<\/span>\u591a\u805a\u7532\u919b1mL<\/span>\uff0c\u56fa\u5b9a15min<\/span>\uff0c\u5f03\u56fa\u5b9a\u6db2\u3002PBS\u6d17\u6da4\u3002\u6bcf\u5b54\u52a0\u51651mL 0.1%<\/span>\u7ed3\u6676\u7d2b\u6216Giemsa<\/span>\u67d3\u8272\u6db2\u67d3\u827210-30min<\/span>\uff0c\u6d41\u6c34\u7f13\u6162\u6d17\u53bb\u67d3\u6db2\uff0c\u7a7a\u6c14\u5e72\u71e5\u3002<\/p>\n

\u8ba1\u6570<\/h3>\n

\u00a0\u00a0 - <\/span>\u663e\u5fae\u955c\u4e0b\u8ba1\u6570\u542b50<\/span>\u4e2a\u7ec6\u80de\u4ee5\u4e0a\u7684\u514b\u9686\u6570\u5e76\u62cd\u7167\uff0c\u53d63<\/span>\u4e2a\u590d\u5b54\u7684\u514b\u9686\u5e73\u5747\u503c\u8fdb\u884c\u8ba1\u7b97\u3002<\/p>\n

\u00a0\u00a0 - <\/span>\u514b\u9686\u5f62\u6210\u7387 =<\/span>\uff08\u514b\u9686\u6570 \/ <\/span>\u63a5\u79cd\u7ec6\u80de\u6570\uff09\u00d7 100%<\/span><\/p>\n

\u7ed3\u679c\u5c55\u793a<\/h3>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u56fe\u7247\u6765\u6e90\u7f51\u7edc\uff0c\u4fb5\u5220<\/p>\n

\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

\"\"<\/p>\n

\u56fe\u7247\u6765\u6e90\u7f51\u7edc\uff0c\u4fb5\u5220<\/p>\n

\u00a0<\/span>\u5236\u5907\u7ec6\u80de\u60ac\u6db2<\/h3>\n

\u00a0\u00a0 - <\/span>\u5c06\u5bf9\u6570\u751f\u957f\u671f\u7684\u5355\u5c42\u7ec6\u80de\u5e38\u89c4\u6d88\u5316\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\uff0c\u91cd\u60ac\u7ec6\u80de\u6c89\u6dc0\uff0c\u8ba1\u6570\uff0c\u7a00\u91ca\u81f31\u00d710\u00b3<\/span>\u4e2a\/mL<\/span>\u3002<\/p>\n

\u914d\u7f6e\u743c\u8102<\/h3>\n

\u00a0\u00a0 - <\/span>\u7528\u84b8\u998f\u6c34\u5206\u522b\u5236\u5907\u51fa1.2%<\/span>\u548c0.7%<\/span>\u4e24\u4e2a\u6d53\u5ea6\u7684\u4f4e\u7194\u70b9\u743c\u8102\u7cd6\u6eb6\u6db2\uff0c\u9ad8\u538b\u706d\u83cc\u540e\uff0c\u7ef4\u6301\u572840\u2103<\/span>\u9632\u6b62\u51dd\u56fa\u3002<\/p>\n

\u00a0\u00a0 - <\/span>\u94fa\u4e0b\u80f6\uff1a\u63091:1<\/span>\u6bd4\u4f8b\u6df7\u54081.2%<\/span>\u743c\u8102\u7cd6\u548c2\u00d7<\/span>\u5b8c\u5168\u57f9\u517b\u57fa\uff08\u542b2\u00d7<\/span>\u6297\u751f\u7d20\u548c20%<\/span>\u80ce\u725b\u8840\u6e05\uff09\uff0c\u53d61.5mL<\/span>\u52a0\u51656<\/span>\u5b54\u677f\u4e2d\uff0c\u51b7\u5374\u51dd\u56fa\uff0c\u4f5c\u5e95\u5c42\u743c\u8102\u7f6e\u4e8e\u57f9\u517b\u7bb1\u4e2d\u5907\u7528\u3002\u4f5c\u4e3a\u652f\u6491\u9632\u6b62\u7ec6\u80de\u8d34\u9644\u751f\u957f\u3002<\/p>\n

\u00a0<\/span>\u63a5\u79cd\u7ec6\u80de<\/h3>\n

\u00a0\u00a0 - <\/span>\u94fa\u4e0a\u80f6\uff1a\u63091:1<\/span>\u6bd4\u4f8b\u6df7\u54080.7%<\/span>\u743c\u8102\u7cd6\u548c2\u00d7<\/span>\u5b8c\u5168\u57f9\u517b\u57fa\uff0c\u518d\u5411\u6df7\u5408\u6db2\u4e2d\u52a0\u51650.2mL<\/span>\u7ec6\u80de\u60ac\u6db2\uff0c\u5145\u5206\u6df7\u5300\uff0c\u6ce8\u5165\u94fa\u67091.2%<\/span>\u743c\u8102\u7cd6\u5e95\u5c42\u7684\u5b54\u677f\u4e2d\uff0c\u5f62\u6210\u53cc\u743c\u8102\u5c42\u3002\u6700\u540e\u52a0\u4e00\u5c42\u57f9\u517b\u57fa\u4ee5\u9632\u5e72\u71e5\u5e76\u8865\u5145\u8425\u517b\u3002\u4e0a\u5c42\u80f6\u7ec6\u80de\u6570\u76ee\u6839\u636e\u4e0d\u540c\u7684\u7ec6\u80de\u7c7b\u578b\u800c\u5b9a\u3002\u901a\u5e38\uff0c\u4f7f\u75285000\u4e2a\u7ec6\u80de\/\u5b54\u4f5c\u4e3a\u8d77\u59cb\u6d53\u5ea6\uff0c\u4f46\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8fdb\u884c\u8c03\u6574\u3002\u4f5c\u4e3a\u8425\u517b\u8865\u5145\u5242\u4ee5\u53ca\u4e3a\u9632\u6b62\u4e0b\u5c42\u743c\u8102\u80f6\u5e72\u71e5\u3002<\/p>\n

\u00a0<\/span>\u57f9\u517b<\/h3>\n

\u00a0\u00a0 - <\/span>\u5ba4\u6e29\u9759\u7f6e30 min<\/span>\uff0c\u5f85\u4e0a\u5c42\u743c\u8102\u51dd\u56fa\u540e\uff0c\u7f6e\u4e8e\u57f9\u517b\u7bb1\u4e2d\u57f9\u517b2-3<\/span>\u5468\u3002<\/p>\n

\u00a0<\/span>\u89c2\u5bdf\u4e0e\u8ba1\u6570<\/h3>\n

\u00a0\u00a0 - <\/span>\u4ee5\u8089\u773c\u53ef\u89c1\u7684\u7ec6\u80de\u56e2\u4f5c\u4e3a\u8ba1\u6570\u96c6\u843d\u7684\u6807\u51c6\uff0c\u8ba1\u7b97\u96c6\u843d\u5f62\u6210\u7387\u3002<\/p>\n

\u00a0<\/span><\/p>\n

Image J<\/span>\u8f6f\u4ef6\u8ba1\u6570\u65b9\u6cd5\uff1a<\/h2>\n
    \n
  • \u70b9\u51fbFile\u2192Open\u2192<\/span>\u6253\u5f00\u56fe\u7247\u3002\uff08\u4e5f\u53ef\u5c06\u56fe\u7247\u76f4\u63a5\u62d6\u52a8\u5230\u83dc\u5355\u680f\uff0c\u5373\u53ef\u6253\u5f00\u3002\uff09<\/li>\n
  • \u5c06\u56fe\u50cf\u8f6c\u4e3a8-bit<\/span>\n
      \n
    • \u70b9\u51fbImage\u2192Type\u21928-bit\uff0c\u6b64\u65f6\u56fe\u7247\u5373\u5df2\u88ab\u53bb\u8272\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n
        \n
      • \u8c03\u6574\u9608\u503c\uff0c\u53bb\u9664\u80cc\u666f\uff0c\u9009\u4e2d\u7ec6\u80de<\/li>\n<\/ul>\n

        \u70b9\u51fbImage\u2192Adjust\u2192Threshold<\/span>\u5bf9\u9608\u503c\u8fdb\u884c\u8c03\u6574\uff0c\u4e3b\u8981\u662f\u4e3a\u4e86\u9009\u62e9\u7ec6\u80de\u533a\u57df\uff1b<\/p>\n

        \u9009\u62e9B&W<\/span>\uff08Black and White<\/span>\uff09\uff0c\u56fe\u50cf\u4e2d\u9ed1\u8272\u7684\u533a\u57df\u5c31\u662f\u5df2\u7ecf\u9009\u4e2d\u7684\u533a\u57df\u3002\uff08\u8fd9\u91cc\u53ef\u4ee5\u8fdb\u884c\u66f4\u6539\uff0c\u5982\u82e5\u6539\u4e3ared<\/span>\uff0c\u5219\u7ea2\u8272\u533a\u57df\u4e3a\u9009\u4e2d\u7684\u533a\u57df\u3002\uff09<\/p>\n

        \u53e6\u5916\u6211\u4eec\u53d1\u73b0Threshold<\/span>\u7a97\u53e3\u4e2d\u6709\u4e24\u4e2a\u8c03\u8282\u6846\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u5de6\u53f3\u62d6\u52a8\u8c03\u8282\u6846\u4e2d\u7684\u6ed1\u5757\u5bf9\u9009\u62e9\u7684\u533a\u57df\u8fdb\u884c\u8c03\u6574\u3002\u539f\u5219\u662f\u5c3d\u53ef\u80fd\u7684\u5305\u542b\u6240\u6709\u7684\u7ec6\u80de\u540c\u65f6\u53bb\u9664\u80cc\u666f\u4e2d\u7684\u6742\u8d28\u3002<\/p>\n

        \u62d6\u52a8\u5b8c\u6210\u540e\u70b9\u51fbApply<\/span>\uff0c\u8fd9\u6837\u9608\u503c\u5c31\u8bbe\u7f6e\u597d\u4e86\u3002<\/p>\n

          \n
        • \u82e5\u51fa\u73b0\u7ec6\u80de\u91cd\u53e0\u6216\u8005\u8d34\u8fd1\u7684\u60c5\u51b5\uff0c\u8f6f\u4ef6\u8bc6\u522b\u65f6\u5c06\u4e24\u4e2a\u7c98\u8fde\u5728\u4e00\u8d77\u7684\u7ec6\u80de\u8bc6\u522b\u4e3a\u4e00\u4e2a\uff0c\u8fd9\u65f6\u53ef\u4ee5\u5229\u7528Watershed<\/span>\u81ea\u52a8\u8bc6\u522b\u91cd\u53e0\u90e8\u5206\uff0c\u968f\u540e\u518d\u5c06\u4e24\u4e2a\u7ec6\u80de\u5206\u79bb\u5f00\u6765\u3002<\/li>\n<\/ul>\n

          \u70b9\u51fbProcess\u2192Binary\u2192Watershed<\/span>\uff0c\u8fd9\u6837\u7c98\u8fde\u7684\u7ec6\u80de\u4f1a\u88ab\u5206\u5f00\u3002<\/p>\n

            \n
          • \u8ba1\u6570\u9897\u7c92<\/li>\n<\/ul>\n

            \u70b9\u51fbAnalyze\u2192Analyze Particles<\/span>\uff0c\u51fa\u73b0Analyze Particles<\/span>\u754c\u9762\uff0c\u6839\u636e\u5904\u7406\u56fe\u50cf\u7684\u4e0d\u540c\uff0c\u8bbe\u7f6e\u4e0d\u540c\u7684\u53c2\u6570\u3002<\/p>\n

            Size<\/span>\uff1a0.05-Infinity\u2014\u2014<\/span>\u6307\u5206\u6790\u9897\u7c92\u5c3a\u5bf8\u5927\u4e8e0.05<\/span><\/p>\n

            Circularity<\/span>\uff1a0.00-1.00\u2014\u2014<\/span>\u6307\u5706\u5ea6\uff0c\u53ef\u4ee5\u6839\u636e\u7ec6\u80de\u5f62\u72b6\uff0c\u8c03\u6574\u9700\u8981\u7684\u5706\u5ea6\uff0c1.00<\/span>\u4e3a\u6807\u51c6\u5706\uff0c\u4e00\u822c\u60c5\u51b5\u4e0b\u53ea\u6839\u636eSize<\/span>\u8fdb\u884c\u9650\u5236\u5c31\u53ef\u4ee5\u4e86\u3002<\/p>\n

            Show<\/span>\uff1aOverlay\u2014\u2014<\/span>\u6307\u539f\u672c\u56fe\u7247\u4e0a\u4f1a\u5c55\u793a\u51fa\u5206\u6790\u7ed3\u679c\u7684\u5916\u6846\u3002<\/p>\n

            Exclude on edges<\/span>\uff1a\u2014\u2014<\/span>\u5904\u4e8e\u8fb9\u7f18\u7684\u9897\u7c92\u4e0d\u8ba1\u5165\u3002<\/p>\n

            \u00a0<\/span>

            \u6ce8\u610f\u4e8b\u9879<\/p>

            <\/div><\/div><\/h1>\n
              \n
            • \u7f13\u51b2\u6db2\u6e05\u6d17\u548c\u56fa\u5b9a\u65f6\u6cbf\u7740\u5b54\u677f\u4fa7\u58c1\u52a0\u5165\uff0c\u4e0d\u8981\u5439\u6389\u7ec6\u80de\u3002<\/li>\n
            • \u7ec6\u80de\u60ac\u6db2\u4e2d\u5355\u4e2a\u5206\u6563\u7ec6\u80de\u5e94\u591a\u4e8e90%<\/span>\uff0c\u5e73\u677f\u57f9\u517b\u65e9\u671f\u5c3d\u4e0d\u8981\u6643\u52a8\u57f9\u517b\u76bf\uff0c\u4ee5\u665a\u7ec6\u80de\u8131\u843d\uff0c\u5bfc\u81f4\u5b9e\u9a8c\u8bef\u5dee\u589e\u52a0\u3002<\/li>\n
            • \u67d3\u8272\u524d\u56fa\u5b9a\u6db2\u8981\u5438\u5e72\u51c0\uff0c\u907f\u514d\u5c40\u90e8\u6b8b\u7559\u56fa\u5b9a\u6db2\u5c06\u67d3\u5242\u7a00\u91ca\u9020\u6210\u67d3\u8272\u4e0d\u5747\u4e00\u3002<\/li>\n
            • \u67d3\u8272\u5b8c\u6210\u540e\uff0c\u52a1\u5fc5\u5c06\u67d3\u6db2\u6e05\u6d17\u5e72\u51c0\uff0c\u4e0d\u8981\u5728\u5b54\u4e2d\u6709\u6b8b\u7559\u3002<\/li>\n<\/ul>\n

              \u00a0<\/span><\/p>\n

              \u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

              - <\/span>\u7ec6\u80de\u6210\u56e2\uff1a<\/strong>\u5236\u5907\u5355\u7ec6\u80de\u60ac\u6db2\u65f6\u9700\u5145\u5206\u5439\u6253\u5206\u6563\u7ec6\u80de\u3002<\/p>\n

              - <\/span>\u63a5\u79cd\u5bc6\u5ea6\u8fc7\u9ad8\uff1a<\/strong>\u4f1a\u5bfc\u81f4\u591a\u4e2a\u514b\u9686\u878d\u5408\uff0c\u5f71\u54cd\u8ba1\u6570\u7ed3\u679c\u3002\u9700\u9884\u5b9e\u9a8c\u786e\u5b9a\u6700\u4f73\u63a5\u79cd\u5bc6\u5ea6\u3002<\/p>\n

              - <\/span>\u67d3\u8272\u4e0d\u5747\u5300\uff1a<\/strong>\u67d3\u8272\u6db2\u8981\u5145\u5206\u8986\u76d6\u7ec6\u80de\uff0c\u67d3\u8272\u65f6\u95f4\u63a7\u5236\u9002\u5f53\u3002<\/p>\n

              - <\/span>\u57f9\u517b\u57fa\u66f4\u6362\uff1a<\/strong>\u5b9a\u671f\u89c2\u5bdf\u57f9\u517b\u57fa\u7684\u989c\u8272\u53ca\u6c61\u67d3\u60c5\u51b5\uff0c\u5fc5\u8981\u65f6\u66f4\u6362\u3002<\/p>\n

              \u00a0<\/span>\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

              -\u00a0<\/span>\u743c\u8102\u6e29\u5ea6\uff1a<\/strong>\u743c\u8102\u4e0e\u57f9\u517b\u57fa\u6df7\u5408\u65f6\u6e29\u5ea6\u4e0d\u5b9c\u8d85\u8fc740\u2103<\/span>\uff0c\u4ee5\u514d\u70eb\u6b7b\u7ec6\u80de\u3002<\/p>\n

              - <\/strong><\/span>\u64cd\u4f5c\u901f\u5ea6\uff1a<\/strong>\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9700\u8fc5\u901f\uff0c\u4ee5\u9632\u5c40\u90e8\u7ed3\u5757\u3002<\/p>\n

              -\u00a0<\/span>\u7ec6\u80de\u5bc6\u5ea6\uff1a<\/strong>\u4e0a\u5c42\u743c\u8102\u4e2d\u7684\u7ec6\u80de\u5bc6\u5ea6\u9700\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u8c03\u6574\uff0c\u4e00\u822c\u4ee5\u6bcf\u5b54200<\/span>\u4e2a\u7ec6\u80de\u4e3a\u5b9c\u3002<\/p>\n

              -\u00a0<\/span>\u743c\u8102\u6bd2\u6027\uff1a<\/strong>\u743c\u8102\u7ecf\u706d\u83cc\u540e\u5e94\u5206\u88c5\u5907\u7528\uff0c\u53cd\u590d\u52a0\u70ed\u6613\u5bfc\u81f4\u743c\u8102\u964d\u89e3\u800c\u4ea7\u751f\u6bd2\u6027\uff0c\u4e14\u5176\u786c\u5ea6\u4e5f\u4f1a\u6709\u6240\u4e0b\u964d\uff0c\u56e0\u6b64\u9ad8\u538b\u706d\u83cc\u540e\u5e94\u8fdb\u884c\u5206\u88c5\u3002<\/p>\n

              \u53ea\u8c08\u7ecf\u9a8c<\/p>

              <\/div><\/div><\/h1>\n

              \u62cd\u7167\u65f6\uff0c\u52a1\u5fc5\u4f7f\u6bcf\u4e2a\u5b54\u4e2d\u65e0\u9634\u5f71\uff0c\u5982\u65e0\u6cd5\u8fbe\u5230\uff0c\u5219\u9010\u4e2a\u5b54\u8fdb\u884c\u62cd\u7167\u3002<\/p>\n

              \u7ec6\u80de\u8ba1\u6570\u65f6\u5efa\u8bae\u591a\u8ba1\u6570\u51e0\u6b21\u3002<\/p>\n

              \u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

              -\u00a0<\/span>\u5747\u5300\u63a5\u79cd\uff1a<\/strong>\u786e\u4fdd\u7ec6\u80de\u63a5\u79cd\u5747\u5300\u662f\u5b9e\u9a8c\u6210\u529f\u7684\u5173\u952e\u3002<\/p>\n

              -\u00a0<\/span>\u51cf\u5c11\u6270\u52a8\uff1a<\/strong>\u57f9\u517b\u8fc7\u7a0b\u4e2d\u4e0d\u8981\u9891\u7e41\u79fb\u52a8\u57f9\u517b\u76bf\uff0c\u4ee5\u514d\u7ec6\u80de\u8131\u843d\u3002<\/p>\n

              - <\/strong><\/span>\u9002\u91cf\u67d3\u8272\uff1a<\/strong>\u67d3\u8272\u65f6\u6ce8\u610f\u65f6\u95f4\u548c\u67d3\u8272\u6db2\u7684\u7528\u91cf\uff0c\u786e\u4fdd\u67d3\u8272\u5747\u5300\u3002<\/p>\n

              \u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n

              -\u00a0<\/span>\u6e29\u5ea6\u63a7\u5236\uff1a<\/strong>\u743c\u8102\u4e0e\u7ec6\u80de\u6df7\u5408\u65f6\u6e29\u5ea6\u8981\u9002\u4e2d\uff0c\u64cd\u4f5c\u8981\u5feb\uff0c\u907f\u514d\u5c40\u90e8\u7ed3\u5757\u3002<\/p>\n

              - <\/strong><\/span>\u51dd\u56fa\u540e\u64cd\u4f5c\uff1a<\/strong>\u786e\u4fdd\u4e0a\u4e0b\u5c42\u743c\u8102\u5b8c\u5168\u51dd\u56fa\u540e\u518d\u8fdb\u884c\u4e0b\u4e00\u6b65\u64cd\u4f5c\u3002<\/p>\n

              -\u00a0<\/span>\u5408\u9002\u5bc6\u5ea6\uff1a<\/strong>\u9009\u62e9\u5408\u9002\u7684\u7ec6\u80de\u5bc6\u5ea6\uff0c\u786e\u4fdd\u7ed3\u679c\u51c6\u786e\u3002<\/p>\n

              \u00a0<\/span>

              \u5e38\u89c1\u95ee\u9898<\/p>

              <\/div><\/div><\/h1>\n

              \u7ec6\u80de\u672a\u5f62\u6210\u514b\u9686\uff1a\u5e76\u975e\u6bcf\u79cd\u7ec6\u80de\u90fd\u53ef\u4ee5\u5f62\u6210\u514b\u9686\uff0c\u514b\u9686\u5f62\u6210\u7387\u4e0e\u7ec6\u80de\u672c\u8eab\u589e\u6b96\u80fd\u529b\u6709\u5173\u3002\u8fd8\u4e0e\u63a5\u79cd\u7ec6\u80de\u5bc6\u5ea6\uff0c\u52a0\u5165\u57f9\u517b\u6db2\u7684\u4f53\u79ef\u3001\u8840\u6e05\u6d53\u5ea6\u6709\u5173\u3002\u56e0\u8be5\u5b9e\u9a8c\u5904\u7406\u65f6\u95f4\u8f83\u957f\uff0c\u5e94\u7ed9\u7ec6\u80de\u591a\u52a0\u57f9\u517b\u57fa\uff0c\u5982\u6bcf\u5b544ml<\/span>\uff0c\u6216\u80053<\/span>\u5929\u66f4\u6362\u4e00\u6b21\u57f9\u517b\u57fa\uff0c\u4ee5\u4f9b\u7ed9\u7ec6\u80de\u5145\u8db3\u7684\u8425\u517b\u3002<\/p>\n

              \u00a0<\/span>\u00a0<\/span>\u5e73\u677f\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n
                \n
              1. \u7ec6\u80de\u6210\u56e2\uff1a<\/strong>\u5236\u5907\u5355\u7ec6\u80de\u60ac\u6db2\u65f6\u9700\u5145\u5206\u5439\u6253\u5206\u6563\u7ec6\u80de\u3002<\/li>\n
              2. \u63a5\u79cd\u5bc6\u5ea6\u8fc7\u9ad8<\/strong>\uff1a\u4f1a\u5bfc\u81f4\u591a\u4e2a\u514b\u9686\u878d\u5408\uff0c\u5f71\u54cd\u8ba1\u6570\u7ed3\u679c\u3002\u9700\u9884\u5b9e\u9a8c\u786e\u5b9a\u6700\u4f73\u63a5\u79cd\u5bc6\u5ea6\u3002<\/li>\n
              3. \u67d3\u8272\u4e0d\u5747\u5300\uff1a<\/strong>\u67d3\u8272\u6db2\u8981\u5145\u5206\u8986\u76d6\u7ec6\u80de\uff0c\u67d3\u8272\u65f6\u95f4\u63a7\u5236\u9002\u5f53\u3002<\/li>\n<\/ol>\n

                \u00a0<\/span>\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5b9e\u9a8c<\/h2>\n
                  \n
                1. \u743c\u8102\u6e29\u5ea6\u8fc7\u9ad8\u6216\u8fc7\u4f4e\uff1a<\/strong>\u8fc7\u9ad8\u4f1a\u70eb\u6b7b\u7ec6\u80de\uff0c\u8fc7\u4f4e\u4f1a\u5bfc\u81f4\u5c40\u90e8\u7ed3\u5757\u3002\u743c\u8102\u4e0e\u57f9\u517b\u57fa\u6df7\u5408\u65f6\u6e29\u5ea6\u4e0d\u5b9c\u8d85\u8fc740\u2103<\/span>\u3002<\/li>\n
                2. \u7ec6\u80de\u5bc6\u5ea6\u4e0d\u5408\u9002\uff1a<\/strong>\u7ec6\u80de\u5bc6\u5ea6\u8fc7\u9ad8\u6216\u8fc7\u4f4e\u90fd\u4f1a\u5f71\u54cd\u7ed3\u679c\uff0c\u9700\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u8c03\u6574\u5bc6\u5ea6\u3002<\/li>\n
                3. \u64cd\u4f5c\u901f\u5ea6\u6162\uff1a<\/strong>\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9700\u8fc5\u901f\uff0c\u4ee5\u9632\u743c\u8102\u5c40\u90e8\u51dd\u56fa\u3002<\/li>\n<\/ol>\n

                  \u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u5931\u8d25\u7684\u539f\u56e0\uff1a\u7ec6\u80de\u81ea\u8eab\u7684\u56e0\u7d20\uff0c\u534a\u56fa\u4f53\u73af\u5883\u8981\u6c42\u7ec6\u80de\u6076\u6027\u7a0b\u5ea6\u9ad8\uff0c\u5bb9\u6613\u751f\u957f\uff0c\u6076\u6027\u7a0b\u5ea6\u4f4e\u4e14\u4f9d\u8d56\u8d34\u58c1\u751f\u957f\u7684\u7ec6\u80de\u53ef\u80fd\u4e0d\u9002\u7528\u4e8e\u6b64\u6cd5\u3002<\/p>\n

                  \u901a\u8fc7\u4ee5\u4e0a\u8be6\u7ec6\u6b65\u9aa4\u548c\u6ce8\u610f\u4e8b\u9879\uff0c\u53ef\u4ee5\u6709\u6548\u5730\u8fdb\u884c\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\uff0c\u4ece\u800c\u8bc4\u4f30\u7ec6\u80de\u7684\u589e\u6b96\u80fd\u529b\u548c\u7fa4\u4f53\u4f9d\u8d56\u6027\u3002<\/p>\n

                  \u53c2\u8003\u6587\u732e\uff1a<\/strong><\/h2>\n

                  Wang J, Zhang N, Han Q, et al. Pin1 inhibition reverses the acquired resistance of human hepatocellular carcinoma cells to Regorafenib via the Gli1\/Snail\/E-cadherin pathway. Cancer Lett. 2019;444:82-93. doi:10.1016\/j.canlet.2018.12.010IF: 9.1\u00a0Q1<\/span><\/p>\n

                  Gan Y, Deng J, Hao Q, et al. UTP11 deficiency suppresses cancer development via nucleolar stress and ferroptosis. Redox Biol. 2023;62:102705. doi:10.1016\/j.redox.2023.102705IF: 10.7\u00a0Q1<\/span><\/p>\n

                  Xu M, Chen X, Lin K, et al. The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p. Mol Cancer. 2018;17(1):141.<\/span><\/p>\n

                  Singh M, Bansal S, Kundu S, et al. Synthesis, Structure-Activity Relationship, and Mechanistic Investigation of Lithocholic Acid Amphiphiles for Colon Cancer Therapy. Medchemcomm. 2015;6(1):192-201. <\/span><\/p>\n

                  Baldeyron C, Brisson A, Tesson B, et al. TIPIN depletion leads to apoptosis in breast cancer cells. Mol Oncol. 2015;9(8):1580-1598.<\/span><\/p>\n

                  Willmer T, Cooper A, Sims D, Govender D, Prince S. The T-box transcription factor 3 is a promising biomarker and a key regulator of the oncogenic phenotype of a diverse range of sarcoma subtypes. Oncogenesis. 2016;5(2):e199.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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