{"id":572,"date":"2024-07-16T09:26:06","date_gmt":"2024-07-16T09:26:06","guid":{"rendered":"https:\/\/yunshangtulv.com.cn\/?p=572"},"modified":"2024-07-16T09:26:06","modified_gmt":"2024-07-16T09:26:06","slug":"%e7%bb%86%e8%83%9e%e5%88%92%e7%97%95%e5%ae%9e%e9%aa%8c","status":"publish","type":"post","link":"https:\/\/yunshangtulv.com.cn\/?p=572","title":{"rendered":"\u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c"},"content":{"rendered":"<h2>\u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c\u6559\u7a0b<\/h2>\n<h3>\u5168\u79f0\u522b\u79f0<\/h3>\n<p>\u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c\uff08Cell scratch assay\uff09\uff0c\u53c8\u79f0\u4f24\u53e3\u6108\u5408\u5b9e\u9a8c\uff08Wound healing 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style=\"font-size: 16px;\">\u4f18\u70b9\uff1a<\/strong><\/p>\n<section>\n<section style=\"padding-left: 40px;\"><strong>\u2460<\/strong>\uff1a\u5728\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u6a21\u62df\u4e86\u4f53\u5185\u7ec6\u80de\u8fc1\u79fb\u7684\u8fc7\u7a0b\u3002<\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2461<\/strong>\uff1a\u975e\u5e38\u9002\u5408\u7814\u7a76\u7ec6\u80de\u4e0e\u80de\u5916\u57fa\u8d28(ECM),\u7ec6\u80de\u4e0e\u7ec6\u80de\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u5f15\u8d77\u7684\u7ec6\u80de\u8fc1\u79fb\u3002<\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2462<\/strong>\uff1a\u4e0e\u5305\u62ec\u6d3b\u7ec6\u80de\u6210\u50cf\u5728\u5185\u7684\u663e\u5fae\u955c\u7cfb\u7edf\u517c\u5bb9,\u53ef\u7528\u4e8e\u5206\u6790\u7ec6\u80de\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2463<\/strong>\uff1a\u7814\u7a76\u7ec6\u80de\u8fc1\u79fb\u7684\u4f53\u5916\u5b9e\u9a8c\u4e2d\u6700\u7b80\u5355\u7684\u65b9\u6cd5\u3002<\/section>\n<section><strong>\u7f3a\u70b9\uff1a<\/strong><\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2460<\/strong>\uff1a\u624b\u52a8\u5212\u75d5\uff0c\u4e0d\u80fd\u4fdd\u8bc1\u6bcf\u6b21\u5212\u75d5\u4e00\u81f4\u3002<\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2461<\/strong>\uff1a\u6709\u65f6\u5019\u5728\u5212\u7ec6\u80de\u7684\u540c\u65f6\u4f1a\u522e\u574f\u4e00\u7247\u7ec6\u80de\uff0c\u5bf9\u5212\u75d5\u7684\u8fb9\u7f18\u7684\u7ec6\u80de\u9020\u6210\u673a\u68b0\u635f\u4f24\u3002<\/section>\n<section style=\"padding-left: 40px;\"><strong>\u2462<\/strong>\uff1a\u5982\u679c\u57f9\u517b\u76bf\u5e95\u90e8\u8fd8\u6709\u5305\u88ab\u86cb\u767d\u7684\u8bdd\uff0c\u67aa\u5934\u5212\u8fc7\uff0c\u5f88\u6709\u53ef\u80fd\u4f24\u5bb3\u5230\u5305\u88ab\uff0c\u8fdb\u800c\u5f71\u54cd\u5230\u7ec6\u80de\u8fc1\u79fb\uff0c\u7ed3\u679c\u4fbf\u5f88\u96be\u5224\u65ad\u662f\u54ea\u4e2a\u56e0\u7d20\u9020\u6210\u4e86\u51b3\u5b9a\u6027\u7684\u5f71\u54cd\u3002<\/section>\n<\/section>\n<h3>\u57fa\u672c\u539f\u7406<\/h3>\n<p>\u5728\u878d\u5408\u7684\u5355\u5c42\u7ec6\u80de\u4e0a\u4eba\u4e3a\u5236\u9020\u4e00\u4e2a\u7a7a\u767d\u533a\u57df\uff08\u5212\u75d5\/\u4f24\u53e3\uff09\uff0c\u5212\u75d5\u8fb9\u7f18\u7684\u7ec6\u80de\u4f1a\u9010\u6e10\u8fdb\u5165\u7a7a\u767d\u533a\u57df\u4f7f\u5212\u75d5\u6108\u5408\u3002\u901a\u8fc7\u6d4b\u91cf\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u5212\u75d5\u95f4\u8ddd\u5e76\u8ba1\u7b97\u5dee\u503c\uff0c\u53ef\u4ee5\u5bf9\u7ec6\u80de\u7684\u8fc1\u79fb\u80fd\u529b\u505a\u51fa\u5224\u65ad\u3002<\/p>\n<h3>\u8bbe\u5907\u8017\u6750<\/h3>\n<ul>\n<li>\u7ec6\u80de\u57f9\u517b\u677f\uff086\u5b54\u677f\uff09<\/li>\n<li>\u663e\u5fae\u955c\uff08\u5e26\u6444\u50cf\u5934\uff09<\/li>\n<li>\u5212\u75d5\u5de5\u5177\uff08200 \u00b5l\u67aa\u5934\u6216\u7ec6\u9488\uff09<\/li>\n<li>\u79fb\u6db2\u5668\u548c\u65e0\u83cc\u5438\u5934<\/li>\n<li>PBS\uff08\u78f7\u9178\u76d0\u7f13\u51b2\u76d0\u6c34\uff09<\/li>\n<li>\u80f0\u86cb\u767d\u9176<\/li>\n<li>\u57f9\u517b\u57fa\uff08DMEM\u6216RPMI-1640\uff0c\u52a0\u516510%\u80ce\u725b\u8840\u6e05\uff09<\/li>\n<li>\u65e0\u8840\u6e05\u57f9\u517b\u57fa<\/li>\n<li>\u9a6c\u514b\u7b14\u3001\u76f4\u5c3a<\/li>\n<\/ul>\n<h3>\u5b9e\u9a8c\u51c6\u5907<\/h3>\n<ol>\n<li><strong>\u6750\u6599\u51c6\u5907<\/strong>\uff1a\u786e\u4fdd\u6240\u6709\u8017\u6750\u548c\u8bbe\u5907\u9f50\u5168\u4e14\u7ecf\u8fc7\u6d88\u6bd2\u72b6\u6001\u826f\u597d\u3002<\/li>\n<li><strong>\u7ec6\u80de\u57f9\u517b<\/strong>\uff1a\u9009\u62e9\u5408\u9002\u7684\u7ec6\u80de\u7ebf\uff0c\u4f7f\u7528\u7279\u5b9a\u57f9\u517b\u57fa\uff0c\u572837\u00b0C\u30015% CO\u2082\u6761\u4ef6\u4e0b\u57f9\u517b\u3002\u5b9e\u9a8c\u524d\u4e00\u5929\u5c06\u7ec6\u80de\u4f20\u81f3\u65b0\u7684\u57f9\u517b\u677f\u4e2d\uff0c\u786e\u4fdd\u5f62\u6210\u7406\u60f3\u7684\u5355\u5c42\u7ec6\u80de\u3002<\/li>\n<li><strong>\u9884\u5904\u7406<\/strong>\uff1a\u5b9e\u9a8c\u524d24\u5c0f\u65f6\u53bb\u9664\u8840\u6e05\uff0c\u4f7f\u7ec6\u80de\u5904\u4e8e\u9759\u6b62\u72b6\u6001\uff0c\u68c0\u67e5\u7ec6\u80de\u5065\u5eb7\u65e0\u6c61\u67d3\uff0c\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\u3002<\/li>\n<\/ol>\n<h3>\u64cd\u4f5c\u6b65\u9aa4<\/h3>\n<p><strong>\u5355\u5c42\u5212\u75d5\u7ec6\u80de\u5236\u5907<\/strong><\/p>\n<ul>\n<li><strong><span>\u7ec6\u80de\u6d88\u5316\uff1a<\/span><\/strong><span>\u4f7f\u7528\u80f0\u86cb\u767d\u9176\u5c06\u51c6\u5907\u7528\u4e8e\u5212\u75d5\u5b9e\u9a8c\u7684\u7ec6\u80de\u8fdb\u884c\u6d88\u5316\uff0c\u79bb\u5fc3-\u5b8c\u5168\u57f9\u517b\u57fa\u91cd\u60ac-\u7ec6\u80de\u8ba1\u6570\u3002<\/span><\/li>\n<li><strong><span>\u79cd\u690d\u7ec6\u80de\uff1a<\/span><\/strong><\/li>\n<\/ul>\n<p><strong>\u5212\u7ebf<\/strong>\uff1a<\/p>\n<ul>\n<li>\u57286\u5b54\u677f\u5e95\u9762\uff0c\u7528\u76f4\u5c3a\u548c\u9a6c\u514b\u7b14\u7ed8\u5236\u5e73\u884c\u7ebf\uff0c\u95f4\u96940.5-1 cm\uff0c\u6bcf\u5b54\u81f3\u5c11\u753b5\u6761\u6c34\u5e73\u7ebf\u3002<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2024\/07\/f591fd83e04629813ee1e86c3cb77b3d521598076.jpg@web-article-pic.avif\" alt=\"\" width=\"235\" height=\"139\" class=\"alignnone size-full wp-image-574\" \/><\/p>\n<ul>\n<li>\u6ce8\u610f\u7ebf\u4e0d\u8981\u592a\u7c97\u3002<\/li>\n<\/ul>\n<p><strong>\u7ec6\u80de\u79cd\u690d<\/strong>\uff1a<\/p>\n<ul>\n<li>\u5c06\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\u7684\u7ec6\u80de\u6d88\u5316\u6210\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u6bcf\u5b54\u63a5\u79cd\u7ea65\u00d710\u2075\u4e2a\u7ec6\u80de\uff08<span>\u4ee56\u5b54\u677f\u4e3a\u4f8b\uff0c\u6bcf\u5b54\u957f\u6ee1\u7ec6\u80de\uff0c\u6570\u91cf\u5927\u6982\u662f1.2 \u00d7 10<\/span><sup><span>6\uff0c<\/span><\/sup><span>\u5efa\u8bae\u79cd\u690d50-70%\u7ec6\u80de\u3002<\/span>\uff09\uff0c\u8fc7\u591c\u57f9\u517b\uff0c\u786e\u4fdd\u7ec6\u80de\u5f62\u6210\u5b8c\u6574\u7684\u5355\u5c42\uff0c\u878d\u5408\u7387\u8fbe\u5230100%\u3002\u3010<span>\u5f53\u7ec6\u80de\u5728\u76bf\u5e95\uff0830 mm \u62166 well\uff09\u878d\u5408\u7387\u8fbe\u523090%\u5de6\u53f3\uff08<\/span><strong><span>\u4e0d\u5efa\u8bae 100% \u6c47\u5408\uff0c\u56e0\u4e3a\u8bb8\u591a\u951a\u5b9a\u4f9d\u8d56\u6027\u7ec6\u80de\u4f1a\u7ecf\u5386\u63a5\u89e6\u6291\u5236\uff0c\u8fd9\u53ef\u80fd\u4f1a\u6539\u53d8\u5b83\u4eec\u7684\u8fc1\u79fb\u80fd\u529b\u3002\u4f4e\u4e8e80%\u4e5f\u4e0d\u884c\uff0c\u5b83\u5bfc\u81f4\u7167\u7247\u65e0\u6cd5\u4f7f\u7528\uff0c\u56e0\u4e3a\u91c7\u96c6\u7684\u56fe\u50cf\u5212\u75d5\u8d77\u4f0f\/\u5f2f\u66f2\u592a\u5927\uff0c\u4e0d\u5229\u4e8e\u5b9e\u9a8c\u6570\u636e\u91c7\u96c6<\/span><\/strong><span>\uff09\u6765\u81eaGBhouse\u5e16\u5b50<\/span>\u3011<\/li>\n<\/ul>\n<p><strong>\u5212\u75d5\u5236\u4f5c<\/strong>\uff1a<\/p>\n<ul>\n<li>\u7528\u76f4\u5c3a\u6bd4\u7740\uff0c\u7528200 \u00b5l\u67aa\u5934\u5782\u76f4\u4e8e\u5b54\u677f\u5e95\u9762\u7684\u6a2a\u7ebf\u5212\u75d5\uff0c\u4f7f\u5212\u75d5\u4e0e\u6807\u8bb0\u7ebf\u76f8\u4ea4\u3002<\/li>\n<li>\u786e\u4fdd\u67aa\u5934\u5782\u76f4\uff0c\u4e0d\u503e\u659c\uff0c\u4e0d\u540c\u5b54\u4e4b\u95f4\u6700\u597d\u4f7f\u7528\u540c\u4e00\u67aa\u5934\uff0c\u4fdd\u6301\u5212\u75d5\u5bbd\u5ea6\u4e00\u81f4\u3002<\/li>\n<li>\u53ef\u4ee5\u4f7f\u7528<strong>ibidi\u5212\u75d5\u63d2\u4ef6\u521b\u5efa\u95f4\u9699\u907f\u514d\u4eba\u5de5\u5212\u75d5<\/strong><\/li>\n<\/ul>\n<p><strong>\u6e05\u6d17\u548c\u5904\u7406<\/strong>\uff1a<\/p>\n<ul>\n<li>\u5212\u75d5\u5b8c\u6210\u540e\uff0c\u7528PBS\u7f13\u6162\u51b2\u6d17\u7ec6\u80de3\u6b21\uff0c\u53bb\u9664\u5212\u4e0b\u7684\u7ec6\u80de\uff0c\u4f7f\u7559\u4e0b\u7684\u95f4\u9699\u6e05\u6670\u53ef\u89c1\u3002<\/li>\n<li>\u7528PBS\u7f13\u51b2\u6db2\u51b2\u6d17\u65f6\uff0c\u6ce8\u610f\u8d34\u58c1\u6162\u6162\u52a0\u5165\uff0c\u4ee5\u514d\u51b2\u6563\u5355\u5c42\u8d34\u58c1\u7ec6\u80de\uff1b\u53cd\u590d\u591a\u6b21\u51b2\u6d17\uff0c\u5c3d\u91cf\u6d17\u6389\u6240\u6709\u7684\u7ec6\u80de\u788e\u7247\uff0c\u5426\u5219\u4f1a\u5f71\u54cd\u62cd\u7167\u6548\u679c\u3002<\/li>\n<li>\u7136\u540e\u52a0\u5165\u65b0\u9c9c\u7684\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u3002\u5982\u679c\u7ec6\u80de\u5bf9\u65e0\u8840\u6e05\u4e0d\u8010\u53d7\uff0c\u53ef\u5c11\u52a0\u70b9\u8840\u6e05\u4ee5\u7ef4\u6301\u7ec6\u80de\u72b6\u6001\u3002<\/li>\n<li>\u5212\u75d5\u7f29\u5c0f\u662f\u7ec6\u80de\u8fc1\u79fb\u548c\u7ec6\u80de\u7e41\u6b96\u5171\u540c\u4f5c\u7528\u7684\u7ed3\u679c\uff0c\u53ef\u7528\u4e1d\u88c2\u9709\u7d20\uff081 \u03bcg\/ml\uff09\u5904\u74061\u5c0f\u65f6\u6291\u5236\u7ec6\u80de\u5206\u88c2\uff0c\u5355\u7eaf\u8bc4\u4f30\u7ec6\u80de\u8fc1\u79fb\u3002<\/li>\n<\/ul>\n<p><strong>\u57f9\u517b\u4e0e\u89c2\u5bdf<\/strong>\uff1a<\/p>\n<ul>\n<li>\u5c06\u57f9\u517b\u677f\u7f6e\u4e8e37\u00b0C\u30015% CO\u2082\u57f9\u517b\u7bb1\u4e2d\u3002\u63090\u30016\u300112\u300124\u300148\u5c0f\u65f6\u7684\u65f6\u95f4\u70b9\u53d6\u51fa\u7ec6\u80de\u89c2\u5bdf\u5e76\u62cd\u7167\u3002<\/li>\n<li>\u4ee5\u9884\u5148\u505a\u597d\u7684\u6807\u8bb0\u4e3a\u5b9a\u70b9\u89c2\u6d4b\u70b9\u6765\u8fdb\u884c\u5b9a\u65f6\u62cd\u7167\u3002<\/li>\n<li>\u9009\u62e9\u5904\u7406\/\u5bf9\u7167\u7ec4\u8fc1\u79fb\u80fd\u529b\u5dee\u5f02\u660e\u663e\u7684\u65f6\u95f4\u70b9\u62cd\u7167\uff0c\u62cd\u6444\u4e0e0h\u62cd\u7167\u65f6\u7684\u76f8\u540c\u4f4d\u7f6e\uff0c\u653e\u5927\u500d\u6570\u76f8\u540c\u3002\u62cd\u7167\u65f6\u6807\u8bb0\u7ebf\u4e0d\u8981\u51fa\u73b0\u5728\u89c6\u91ce\u5185\uff0c\u800c\u662f\u521a\u597d\u6cbf\u7740\u6807\u8bb0\u7ebf\u7684\u8fb9\u62cd\u7167\u3002\u7ec6\u80de\u751f\u957f\u5177\u6709\u8fb9\u7f18\u6548\u5e94\uff0c\u6240\u4ee5\u8fb9\u7f18\u7ec6\u80de\u751f\u957f\u72b6\u6001\u53ef\u80fd\u4e0e\u4e2d\u95f4\u7684\u7ec6\u80de\u4e0d\u592a\u4e00\u6837\uff0c\u4e0d\u5b9c\u4f5c\u4e3a\u89c2\u6d4b\u70b9\u3002<\/li>\n<li>\u67d3\u8272\u548c\u89c2\u5bdf\uff1a\u53ef\u9009\u8fdb\u884c\u7ec6\u80de\u67d3\u8272\uff0c\u5982\u4f7f\u7528Crystal Violet\u67d3\u8272\uff0c\u4ee5\u53ef\u89c6\u5316\u7ec6\u80de\u5728\u5212\u75d5\u533a\u57df\u7684\u8fc1\u79fb\u60c5\u51b5\u3002<\/li>\n<\/ul>\n<p><strong>\u56fe\u50cf\u91c7\u96c6\u3001\u6570\u636e\u5206\u6790<\/strong>\uff1a<\/p>\n<ul>\n<li><span>\u4f7f\u7528 Image J \u8f6f\u4ef6\u6253\u5f00\u56fe\u7247\u540e\uff0c\u968f\u673a\u5212\u53d6 6-8 \u6761\u6c34\u5e73\u7ebf\uff0c\u8ba1\u7b97\u7ec6\u80de\u95f4\u8ddd\u79bb\u7684\u5747\u503c\u3002<\/span><\/li>\n<li>\u4f7f\u7528ImageJ\u8f6f\u4ef6\u5206\u6790\u5212\u75d5\u9762\u79ef\u7684\u53d8\u5316\uff0c\u8ba1\u7b97\u4f24\u53e3\u6108\u5408\u7387\uff1a\n<div class=\"dark bg-gray-950 rounded-md border-[0.5px] border-token-border-medium\">\n<div class=\"overflow-y-auto p-4\" dir=\"ltr\"><code class=\"!whitespace-pre hljs language-scss\">\u4f24\u53e3\u6108\u5408\u7387 (%) = <span class=\"hljs-selector-attr\">[(0 h\u5212\u75d5\u9762\u79ef - 24 h\u5212\u75d5\u9762\u79ef) \/ 0 h\u5212\u75d5\u9762\u79ef]<\/span> \u00d7 <span class=\"hljs-number\">100%<\/span><br \/>\n<\/code><\/div>\n<\/div>\n<\/li>\n<li>\u4f7f\u7528ImageJ\u8f6f\u4ef6\u6d4b\u91cf\u7ec6\u80de\u8fc1\u79fb\u8ddd\u79bb\uff0c\u4ee5P\u503c\u5c0f\u4e8e0.05\u4e3a\u7ed3\u679c\u5177\u6709\u663e\u8457\u6027\uff0c\u4f7f\u7528GraphPad Prism \u8f6f\u4ef6\u7ed8\u5236\u7edf\u8ba1\u56fe\u3002<\/li>\n<li><strong>\u7ec6\u80de\u8fc1\u79fb\u8ddd\u79bb\uff1a<\/strong>\u6bcf\u4e2a\u65f6\u95f4\u70b9\u7684\u8ddd\u79bb-0 h\u8ddd\u79bb=\u6bcf\u4e2a\u65f6\u95f4\u957f\u5ea6\u7ec6\u80de\u6574\u4f53\u8fc1\u79fb\u7684\u8ddd\u79bb\uff0c\u6c42\u51fa\u5747\u6570\u548c\u6807\u51c6\u5dee\u3002\u4ee5\u65f6\u95f4\uff08h\uff09\u4e3a\u6a2a\u8f74\uff0c\u8fc1\u79fb\u8ddd\u79bb\u4e3a\u7eb5\u8f74\uff08\u5355\u4f4dmm\uff09\u4f5c\u56fe\uff0c\u5e76\u6bd4\u8f83\u521d\u59cb 0 h\u4e0e\u5b9e\u9a8c\u7ed3\u675f\u65f6\u5b9e\u9a8c\u7ec4\u4e0e\u5bf9\u7167\u7ec4\u7684\u7167\u7247\u3002<\/li>\n<li><strong>\u8ba1\u7b97\u5212\u75d5\u9762\u79ef\uff1a<\/strong><span>\u9762\u79ef\u68c0\u6d4b\u65b9\u6cd5\uff08\u5212\u75d5\u8ddd\u79bb\u6d4b\u91cf\u4e3a\u7b49\u6548\u6d4b\u91cf\uff09\uff1b<\/span><span>\u5212\u75d5\u5bbd\u5ea6\u5e73\u5747\u503c\u00a0=\u00a0\u5212\u75d5\u7a7a\u9699\u9762\u79ef\/\u957f\u5ea6\uff1b<\/span><span>\u7ec6\u80de\u8fc1\u79fb\u7387\u00a0=<\/span><span>\uff08<\/span><span>0 h\u00a0\u5212\u75d5\u5bbd\u5ea6<\/span><span>-<\/span><span>\u57f9\u517b\u540e\u5212\u75d5<\/span><span>\u5bbd\u5ea6\uff09<\/span><span>\/ 0 h\u00a0\u5212\u75d5\u5bbd\u5ea6\u00d7100%<\/span><\/li>\n<li><b>\u5212\u75d5\u9762\u79ef\u548c\u5212\u75d5\u5bbd\u5ea6\u53c2\u89c1\u9178\u83dc\u77e5\u4e4e\u8d34\uff1a<a href=\"https:\/\/zhuanlan.zhihu.com\/p\/110322855\">\u5982\u4f55\u7528ImageJ\u8fdb\u884c\u5212\u75d5\u5b9e\u9a8c\u5bbd\u5ea6\u5206\u6790\uff1f - \u77e5\u4e4e (zhihu.com)<\/a>\u6216\u79d1\u7814\u4e0d\u6742\u70e9\uff1ahttps:\/\/mp.weixin.qq.com\/s\/GFB_gxlrIFr_tPBU099NvA<\/b><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yunshangtulv.com.cn\/wp-content\/uploads\/2024\/07\/c13ef82be3d6247b0fc11bb4d79989a8521598076.png@1256w_358h_web-article-pic.avif\" alt=\"\" width=\"648\" height=\"184\" class=\"alignnone size-full wp-image-573\" \/><\/p>\n<p>&nbsp;<\/p>\n<h3>\u6ce8\u610f\u4e8b\u9879<\/h3>\n<ol>\n<li><strong>\u5de5\u5177\u6d88\u6bd2<\/strong>\uff1a\u4f7f\u7528\u7d2b\u5916\u7ebf\u5f7b\u5e95\u6d88\u6bd2\u6240\u6709\u63a5\u89e6\u7ec6\u80de\u7684\u5de5\u5177\uff0c\u5982\u76f4\u5c3a\u548c\u9a6c\u514b\u7b14\uff0c\u907f\u514d\u7ec6\u83cc\u6c61\u67d3\u3002<\/li>\n<li><strong>\u7ec6\u80de\u63a5\u79cd\u5bc6\u5ea6\u8c03\u6574<\/strong>\uff1a\u6839\u636e\u7ec6\u80de\u7684\u751f\u957f\u901f\u7387\u8c03\u6574\u63a5\u79cd\u6570\u91cf\uff0c\u786e\u4fdd\u6bcf\u4e2a\u57f9\u517b\u5b54\u7684\u7ec6\u80de\u5bc6\u5ea6\u4e00\u81f4\uff0c\u5747\u5300\u5206\u5e03\u3002<\/li>\n<li><strong>\u4fdd\u6301\u5212\u75d5\u4e00\u81f4\u6027<\/strong>\uff1a\u4f7f\u7528\u6807\u51c6\u5316\u5de5\u5177\u548c\u4e00\u81f4\u7684\u64cd\u4f5c\u65b9\u6cd5\u5236\u4f5c\u5212\u75d5\uff0c\u786e\u4fdd\u6bcf\u6b21\u5b9e\u9a8c\u4e2d\u5212\u75d5\u7684\u5bbd\u5ea6\u548c\u6df1\u5ea6\u4e00\u81f4\u3002<\/li>\n<li><strong>\u8f7b\u67d4\u51b2\u6d17<\/strong>\uff1a\u4f7f\u7528PBS\u7f13\u6162\u51b2\u6d17\u7ec6\u80de\uff0c\u907f\u514d\u5bf9\u7ec6\u80de\u5c42\u9020\u6210\u7834\u574f\u3002<\/li>\n<li><strong>\u63a7\u5236\u7ec6\u80de\u589e\u6b96<\/strong>\uff1a\u4f7f\u7528\u65e0\u8840\u6e05\u6216\u4f4e\u8840\u6e05\u57f9\u517b\u57fa\uff0c\u6216\u7528\u4e1d\u88c2\u9709\u7d20\u6291\u5236\u7ec6\u80de\u5206\u88c2\uff0c\u51cf\u5c11\u589e\u6b96\u5bf9\u5b9e\u9a8c\u7ed3\u679c\u7684\u5f71\u54cd\u3002<\/li>\n<li><strong>\u56fa\u5b9a\u89c2\u5bdf\u70b9<\/strong>\uff1a\u6309\u6807\u8bb0\u7ebf\u7684\u4f4d\u7f6e\u5212\u75d5\uff0c\u786e\u4fdd\u89c2\u5bdf\u548c\u62cd\u7167\u65f6\u4f4d\u7f6e\u56fa\u5b9a\u3002<\/li>\n<li><span>\u80f0\u86cb\u767d\u9176\u4f1a\u5f71\u54cd\u7ec6\u80de\u8868\u9762\u53d7\u4f53\uff0c\u8fdb\u800c\u5f71\u54cd\u7ec6\u80de\u8fc1\u79fb\uff0c\u5efa\u8bae\u63a7\u5236\u597d\u65f6\u95f4<\/span><\/li>\n<li><strong><span>\u62cd\u7167\u6ce8\u610f\u4e8b\u9879<\/span><\/strong><span>\uff0c\u4f7f\u7528\u4f4e\u500d\u7269\u955c\uff0810X\uff09\u786e\u5b9a\u5212\u75d5\u56fe\u50cf\u91c7\u96c6\u4f4d\u7f6e\uff1b\u4fdd\u6301\u5212\u75d5\u5b8c\u5168\u6c34\u5e73\u5e76\u4f4d\u4e8e\u89c6\u91ce\u7684\u4e2d\u5fc3\uff0c\u83b7\u53d6\u5c3d\u53ef\u80fd\u591a\u7684\u56fe\u50cf\u4ee5\u6db5\u76d6\u5212\u75d5\u957f\u5ea6\u7684 90%\uff0c\u5c3d\u91cf\u4e0d\u8981\u5305\u542b\u5212\u75d5\u4e24\u7aef\u76845%\u3002<\/span><\/li>\n<li><strong><span>\u56fe\u50cf\u91c7\u96c6\u65f6\u95f4<\/span><\/strong><span>\uff0c\u4e00\u822c\u662f\u5efa\u8bae0h\u30016h\u300112h\u300124h\u300136h\u300148h\u300160h\u300172h\u91c7\u96c6\u56fe\u50cf\u6570\u636e\uff0c\u66f4\u80fd\u591f\u8f83\u51c6\u786e\u7684\u53cd\u5e94\u7ec6\u80de\u7684\u8fc1\u79fb\u60c5\u51b5\uff0c\u540c\u65f6\u4e5f\u589e\u52a0\u6570\u636e\u91cf\u3002<\/span><\/li>\n<li>\u4e3a\u4e86\u4fdd\u8bc1\u7ec6\u80de\u722c\u8fc7\u53bb\u800c\u4e0d\u662f\u56e0\u4e3a\u7ec6\u80de\u589e\u6b96\u88ab\u6324\u8fc7\u53bb\u7684\uff0c\u9700\u8981\u7528\u4e1d\u88c2\u9709\u7d20\u5904\u7406\uff0c\u8ba9\u7ec6\u80de\u505c\u6b62\u589e\u6b96\uff1b\u6216\u5212\u51fa\u5212\u75d5\u540e\uff0c\u628a\u57f9\u517b\u57fa\u6362\u6210\u65e0\u8840\u6e05\u7684\u57f9\u517b\u57fa\uff08\u6709\u7684\u7ec6\u80de\u5bf9\u65e0\u8840\u6e05\u4e0d\u8010\u53d7\uff0c\u53ef\u4ee5\u5c11\u52a0\u70b9\u8840\u6e05\uff0c\u7ef4\u6301\u7ec6\u80de\u7684\u72b6\u6001\uff09 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Ashby, A. Zijlstra. Established and novel methods of interrogating two-dimensional cell migration. Integr Biol 2012, 10.1039\/c2ib20154b<\/li>\n<li>H. Wu, G. Xiao, X. He, J. Ju, J. Zhang, G. Sathishkumar, L. Yu, K. Zhang, X. Rao, Z. Lu, E.-T. Kang, L. Xu. A Wound Exudate-Activated Yarn Battery for Antimicrobial Electrical Fabric Dressing. Adv. Funct. Mater. 2024, 2405114.<\/li>\n<li>Zhong, D.; Zuo, Y.; Shi, Y.; Zhang, P.; Xu, Y. and Li, B., Right once for all: Zinc-modulated highly stable iron-based ROS generator under physiological conditions for promoting bacteria-infected wound healing, Chem. Eng. J., 2023, 460, 141837.<\/li>\n<li>Jung, S. H.; Jang, B. H.; Kwon, S.; Park, S. J.; Park, T. E. and Kang, J. H., Nematic Fibrin Fibers Enabling Vascularized Thrombus Implants Facilitate Scarless Cutaneous Wound Healing, Adv. Mater., 2023, 35, e2211149.<\/li>\n<li>Peglion, F.; Capuana, L.; Perfettini, I. et al., PTEN inhibits AMPK to control collective migration, Nat Commun. 2022, 13, 4528.<\/li>\n<li>Wei, C.; You, C.; Zhou, L.; Liu, H.; Zhou, S.; Wang, X.; Guo, R., Antimicrobial hydrogel microneedle loading verteporfin promotes skin regeneration by blocking mechanotransduction signaling, Chem. Eng. J., 2023, 472, 144866.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c\u6559\u7a0b \u5168\u79f0\u522b\u79f0 \u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c\uff08Cell scratch assay\uff09\uff0c\u53c8\u79f0\u4f24\u53e3\u6108\u5408\u5b9e\u9a8c\uff08Wound [&hellip;]<\/p>\n","protected":false},"author":111,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[],"class_list":["post-572","post","type-post","status-publish","format-standard","hentry","category-10"],"_links":{"self":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/572","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/users\/111"}],"replies":[{"embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=572"}],"version-history":[{"count":0,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/572\/revisions"}],"wp:attachment":[{"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=572"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yunshangtulv.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}